Majeed Shuja, Shah Bikas R, Khalid Nimra, Bielke Lisa, Nazmi Ali
Department of Animal Sciences, College of Food Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.
Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore 54000, Punjab, Pakistan.
Animals (Basel). 2024 Nov 30;14(23):3463. doi: 10.3390/ani14233463.
At day 21 of age, Ross-308 broilers were orally gavaged with 7.5 × 10 CFU/mL S. Typhimurium (n = 30), and another 30 birds were kept as the control. The body weight of birds was recorded on days 0, 2, 7, and 14 days post-infection (dpi) to calculate body weight gains (BWGs). At each time point, seven birds per group were euthanized for sample collection to acquire IELs and lymphocytes from the ileum and spleen for flow cytometric analysis. A reduction in BWGs of the infected groups compared to the control group was observed only at 2 dpi. Additionally, there were no changes in the expression of , , and in the ileum at 2 and 7 dpi. The number of IELs increased significantly following infection in the ileum at 2 and 7 dpi without any changes in spleen lymphocytes. The increase in the total number of IELs was derived from the elevated numbers of conventional CD8αβTCRαβ and natural IEL populations (CD4CD8TCRαβ, CD8ααTCRαβ, TCRγδ, non-T cells (TCR, and iCD8α cells)). The increase in regulatory IELs and the stable expression of proinflammatory cytokine genes during the first week of infection suggests the potential role of IELs in modulating intestinal inflammation.
在21日龄时，给30只罗斯308肉鸡经口灌胃7.5×10 CFU/mL鼠伤寒沙门氏菌，另外30只鸡作为对照。在感染后第0、2、7和14天记录鸡的体重，以计算体重增加量（BWGs）。在每个时间点，每组处死7只鸡进行样本采集，从回肠和脾脏获取肠上皮内淋巴细胞（IELs）和淋巴细胞用于流式细胞术分析。仅在感染后第2天观察到感染组的体重增加量与对照组相比有所降低。此外，在感染后第2天和第7天，回肠中 、 和 的表达没有变化。在感染后第2天和第7天，回肠中感染后IELs数量显著增加，脾脏淋巴细胞无任何变化。IELs总数的增加源于传统CD8αβTCRαβ和天然IEL群体（CD4CD8TCRαβ、CD8ααTCRαβ、TCRγδ、非T细胞（TCR和iCD8α细胞））数量的增加。感染第一周调节性IELs的增加和促炎细胞因子基因的稳定表达表明IELs在调节肠道炎症中具有潜在作用。
