[经支气管镜获取细胞的染色体非整倍体分析]
[Analysis of chromosomal aneuploidy in bronchoscopically gained cells].
作者信息
Gao Shugeng, Dong Xiangyang, Cheng Shujun, Wang Liangjun, Zhang Dechao
机构信息
Department of Thoracic Surgery, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical University, Beijing 100021, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2002 Aug 25;82(16):1090-2.
OBJECTIVE
To study the feasibility of interphase fluorescence in situ hybridization (FISH) in detection of chromosomal aneuploidy in bronchoscopically gained cells from lung cancer patients and early diagnosis of lung cancer.
METHOD
Cells gained from endobronchial brushings of 14 patients with lung cancer were examined by FISH with chromosomes 7, 8, 9 and 12 specific centromere probes.
RESULT
Aneuploidy, mainly featured in gains of chromosomes 7, 8 and 12 and loss of chromosome 9 were observed in all of the 7 cases with cells from endobronchial brushings diagnosed as or suspicious of cancer cells by cytology. Aneuploidies of chromosomes 7, 8, and 9 were found in 3 of other 7 cases that appeared to be normal by cytologic criteria.
CONCLUSION
Interphase FISH is feasible in detecting aneuploidy associated with malignancy in bronchoscopically gained cells that do not meet the criteria of malignancy by conventional cytologic study. This method may be applied to early diagnosis of lung cancer.
目的
研究间期荧光原位杂交（FISH）技术检测肺癌患者支气管镜获取细胞中染色体非整倍体及肺癌早期诊断的可行性。
方法
用7、8、9和12号染色体特异性着丝粒探针，对14例肺癌患者支气管刷检获得的细胞进行FISH检测。
结果
在7例经细胞学诊断为癌细胞或可疑癌细胞的支气管刷检细胞中，均观察到非整倍体，主要表现为7、8和12号染色体增加及9号染色体丢失。在另外7例细胞学检查看似正常的病例中，有3例发现7、8和9号染色体非整倍体。
结论
间期FISH技术可用于检测支气管镜获取的细胞中与恶性肿瘤相关的非整倍体，这些细胞按传统细胞学检查不符合恶性肿瘤标准。该方法可用于肺癌的早期诊断。
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