[吸烟大鼠气道上皮细胞中核因子-κB和细胞间黏附分子-1的表达]

[Nuclear factor-kappa B and intercellular adhesion molecule-1 expression in airway epithelial cells from smoking rats].

作者信息

Xu Jianying, Du Yongcheng, Li Dongyan, Xu Yongjian

机构信息

Department of Pulmonary Disease, Tongji Hospital, Tongji Medical College, Wuhan 430030, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2002 Aug;25(8):493-6.

PMID:12425838

Abstract

OBJECTIVE

To investigate the changes and of nuclear factor-kappa B (NF-kappaB) and intercellular adhesion molecule (ICAM)-1 expression in airway epithelial cells from smoking rats.

METHODS

Wistar rats (n = 39) were randomly divided into a nonsmoking control group and 2 smoking groups-smoking for 1 month and 3 months, with 13 rats in each group. Immunohistochemistry and in situ hybridization staining were used to semiquantitatively analyse the changes of NF-kappaB and ICAM-1 expression in airway epithelial cells.

RESULTS

(1) The percentages of positive cells for NF-kappaB nuclear staining in bronchiolar epithelial cells in 1 month smoking group (63 +/- 4)% and 3 month smoking group [(51 +/- 5)%] were significantly higher than that in the control group [(27 +/- 5)%] (P < 0.01 respectively). (2) ICAM-1 mRNA expression in bronchial and bronchiolar epithelial cells in the 1 month smoking group (0.645 +/- 0.038, 0.747 +/- 0.041) and the 3 month smoking group (0.688 +/- 0.062, 0.809 +/- 0.023) was significantly higher than those in the control group (0.526 +/- 0.023, 0.635 +/- 0.044), (P < 0.01 respectively), and the expression of ICAM-1 mRNA in the 3 month smoking group was higher than that in the 1 month smoking group (P < 0.05 and P < 0.01 respectively). (3) ICAM-1 protein expression in the main bronchial and bronchiolarepithelial cells in the 1 month smoking group (0.73 +/- 0.04, 0.94 +/- 0.05) and the 3 month smoking group (0.77 +/- 0.04, 0.99 +/- 0.03) was significantly higher than those in the control group (0.57 +/- 0.04, 0.83 +/- 0.04) (P < 0.01 respectively), and the expression of ICAM-1 protein in the 3 month smoking group was higher than those in the 1 month smoking group (P < 0.05 respectively). (4) ICAM-1 mRNA and protein expression in bronchioles were significantly higher than those in the bronchi (P < 0.01 respectively) in all groups. (5) The percentage of cells staining positive for nuclear NF-kappaB correlated with ICAM-1 protein expression in bronchioles from the 1 month smoking group (r = 0.462, P < 0.05).

CONCLUSION

NF-kappaB and ICAM-1 may play an important role in smoking-induced airway inflammation.

摘要

目的

探讨吸烟大鼠气道上皮细胞核因子-κB（NF-κB）及细胞间黏附分子（ICAM）-1表达的变化。

方法

将39只Wistar大鼠随机分为非吸烟对照组和2个吸烟组，即吸烟1个月组和吸烟3个月组，每组13只。采用免疫组织化学和原位杂交染色法半定量分析气道上皮细胞中NF-κB和ICAM-1表达的变化。

结果

（1）吸烟1个月组细支气管上皮细胞中NF-κB核染色阳性细胞百分比为（63±4）%，吸烟3个月组为[（51±5）%]，均显著高于对照组[（27±5）%]（P均<0.01）。（2）吸烟1个月组支气管和细支气管上皮细胞中ICAM-1 mRNA表达（0.645±0.038，0.747±0.041）及吸烟3个月组（0.688±0.062，0.809±0.023）均显著高于对照组（0.526±0.023，0.635±0.044）（P均<0.01），且吸烟3个月组ICAM-1 mRNA表达高于吸烟1个月组（P分别<0.05和<0.01）。（3）吸烟1个月组主支气管和细支气管上皮细胞中ICAM-1蛋白表达（0.73±0.04，0.94±0.05）及吸烟3个月组（0.77±0.04，0.99±0.03）均显著高于对照组（0.57±0.04，0.83±0.04）（P均<0.01），且吸烟3个月组ICAM-1蛋白表达高于吸烟1个月组（P均<0.05）。（4）所有组中细支气管ICAM-1 mRNA和蛋白表达均显著高于支气管（P均<0.01）。（5）吸烟1个月组细支气管中核NF-κB染色阳性细胞百分比与ICAM-1蛋白表达相关（r = 0.462，P<0.05）。