Cui Jin, Chen Pan-bi, Yang Xiao-fang, Huang Hui
Department of Acupuncture and Massage, Guiyang College of TCM, Guiyang 550002, China.
Zhongguo Zhen Jiu. 2010 Feb;30(2):141-5.
To explore the underlying mechanism of the simple acupoint catgut emhedding on the treatment of the asthma in rats.
Thirty two Wistar rats with asthma were estahlished hy ovalhumin (OVA), and randomly divided into four groups: the simple acupoint catgut emhedding group (A), Dexamethasone group (B), model group (C), control group (D), 8 cases in each group. In group A, emhed thread was carried out on the first experimental day, "Feishu (BL 13)" "Danzhong (CV 17)" "Shenshu (BL 23)" were selected. In the group B and the group C, 1.5 mg/kg Dexamethasone and 20 mg/kg sterile water were intraperitoneal injected respectively from the 15th experimental day, once a day for 2 weeks consistently. There was no treatment in the group D. For the four groups, asthmatic attack was ohserved, the cells in hronchoalveolar lavage fluid were counted and classified, the pathologic change of lung tissue was examined, the level of ICAM-1 and NF-kappaB in hronchioles was detected with immunohistochemistry.
(1) In the group A, the symptom of asthmatic attack was alleviated and the infiltration of inflammatory cells around the hronchi were remarkahly decreased. In the group C, the total amount of cells in hronchoalveolar lavage fluid and other inflammatory cells were ohviously higher than those of group A. On the other hand, inflammatory effusion in airway and infiltration of inflammatory cells in airway wall in the group A were lower than those of group C. (2) The expression of ICAM-1 and NP-kappaB in lung tissue of group C was higher than those of group D (P < 0.01). (3) The expression of ICAM-1 and NF-kappaB in lung tissue of group B and group A was ohviously lower than those of group C (P < 0.05, P < 0.01).
The simple acupoint catgut emhedding can inhahit the expression of ICAM-1 and NP-kappaB and alleviate airway inflammation in the asthmatic rats.
探讨单纯穴位埋线治疗大鼠哮喘的潜在机制。
采用卵清蛋白（OVA）建立32只哮喘Wistar大鼠模型，随机分为四组：单纯穴位埋线组（A组）、地塞米松组（B组）、模型组（C组）、对照组（D组），每组8只。A组于实验第1天进行埋线，选取“肺俞（BL 13）”“膻中（CV 17）”“肾俞（BL 23）”。B组和C组从实验第15天起分别腹腔注射1.5mg/kg地塞米松和20mg/kg无菌水，每天1次，连续2周。D组不做处理。观察四组大鼠哮喘发作情况，对支气管肺泡灌洗液中的细胞进行计数和分类，检查肺组织病理变化，采用免疫组化法检测细支气管中ICAM-1和NF-κB的水平。
（1）A组哮喘发作症状减轻，支气管周围炎症细胞浸润明显减少。C组支气管肺泡灌洗液中细胞总数及其他炎症细胞明显高于A组。同时，A组气道内炎性渗出及气道壁炎症细胞浸润低于C组。（2）C组肺组织中ICAM-1和NP-κB的表达高于D组（P<0.01）。（3）B组和A组肺组织中ICAM-1和NF-κB的表达明显低于C组（P<0.05，P<0.01）。
单纯穴位埋线可抑制哮喘大鼠ICAM-1和NP-κB的表达，减轻气道炎症。
