Pérez-López Marcos, Nóvoa-Valiñas M Carme, Melgar-Riol M Julia
Department of Toxicology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain.
Toxicol Lett. 2002 Dec 15;136(2):97-106. doi: 10.1016/s0378-4274(02)00284-9.
At the present work, different groups of rainbow trouts have been intraperitoneally treated with a polychlorinated biphenyls commercial mixture (Arochlor-1254) at three doses (5, 20 and 70 mg PCBs/ kg body weight) and they have been sampled at 0 (injection moment), 3, 6 and 9 weeks after xenobiotic exposure. Purification and characterization of the cytosolic isoforms of glutathione S-transferases from three different rainbow trout organs (liver, kidney and gills) have been developed in order to establish the existence of some enzymatic induction process. According to the enzymatic activity levels and protein content, liver samples showed the highest glutathione S-transferases induction process, and resulting dose and time dependent. Directly related, HPLC results from liver retained-affinity purified samples determined that this process was mainly associated to a pi-class related isoenzyme. Only some of the different GST isoenzymes from rainbow trout liver cytosol showed to be specifically induced by the polychlorinated biphenyls treatment, rendering these isoenzymes as valuable biomarkers for exposure to these environmental pollutants.
在当前的研究工作中，不同组的虹鳟鱼被腹腔注射一种多氯联苯商业混合物（Arochlor - 1254），剂量分别为三种（5、20和70毫克多氯联苯/千克体重），并在接触外源化合物后的0（注射时刻）、3、6和9周进行采样。为了确定是否存在某种酶诱导过程，对来自虹鳟鱼三个不同器官（肝脏、肾脏和鳃）的谷胱甘肽S - 转移酶的胞质同工型进行了纯化和表征。根据酶活性水平和蛋白质含量，肝脏样本显示出最高的谷胱甘肽S - 转移酶诱导过程，且呈剂量和时间依赖性。与之直接相关的是，肝脏保留亲和纯化样本的高效液相色谱结果表明，这一过程主要与一种π类相关同工酶有关。虹鳟鱼肝脏胞质中只有一些不同的谷胱甘肽S - 转移酶同工酶显示出被多氯联苯处理特异性诱导，使这些同工酶成为暴露于这些环境污染物的有价值的生物标志物。
