Division of Aquaculture, Department of Public Health and Animal Science, University of Bari, Str. Prov. per Casamassima km 3, 70010, Valenzano, Italy.
Fish Physiol Biochem. 2012 Oct;38(5):1497-505. doi: 10.1007/s10695-012-9640-2. Epub 2012 Apr 8.
The main goal of this work was to determine the effect of dietary live yeast Saccharomyces cerevisiae on the oxidative status of sea bass Dicentrarchus labrax juveniles. Fishes were fed on three diets: the GM group were fed a diet containing lyophilized yeast grown on grape must, the CS group were fed a diet containing lyophilized yeast grown on cornstarch, and the control group were fed a diet without yeast. The activity of the main antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase, glutathione S-transferase (GST), and glutathione (GSH) content, as well as lipid peroxidation, was measured in the liver of sea bass juveniles 90 days after hatching. Supplementation of the diet with S. cerevisiae significantly reduced the SOD and CAT activity, increased the GST activity, decreased the GSH content, and had no effect on lipid peroxidation. The results support the already reported radical-scavenging properties of yeast and usefulness of its employment as antiperoxidative agent in fish.
本研究的主要目的是确定日粮中添加活酵母 Saccharomyces cerevisiae 对海鲈（Dicentrarchus labrax）幼鱼氧化状态的影响。实验将鱼分为三组进行投喂：GM 组投喂含在葡萄汁中培养的冻干酵母的日粮，CS 组投喂含在玉米淀粉中培养的冻干酵母的日粮，对照组投喂不含酵母的日粮。在孵化后 90 天，测量海鲈幼鱼肝脏中的主要抗氧化酶（超氧化物歧化酶[SOD]、过氧化氢酶[CAT]、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶[GST]和谷胱甘肽[GSH]含量）的活性以及脂质过氧化水平。日粮中添加 S. cerevisiae 可显著降低 SOD 和 CAT 的活性，增加 GST 的活性，降低 GSH 含量，且对脂质过氧化没有影响。这些结果支持酵母具有清除自由基的特性，并证明其可用作鱼类的抗氧化剂。
