Hodges G M, Muir M D
J Microsc. 1975 Jul;104(2):173-8. doi: 10.1111/j.1365-2818.1975.tb04014.x.
Measurements of mass of developed silver in autoradiograms have been made using X-ray microanalysis. This procedure has been applied to a scanning electron microscopic study of protease-induced cell stimulation in 3H-thymidine-labelled bladder tissues in vitro. Differences in silver concentrations over individual nuclei were demonstrated by X-ray analysis and quantitative data was obtained showing an increased rate of 3H-thymidine incorporation in protease-treated cultures. The initial evidence indicates that X-ray microanalysis could provide a potentially useful quantitative procedure for autoradiography.
已使用X射线微量分析对放射自显影片中显影银的质量进行了测量。该方法已应用于体外3H-胸腺嘧啶核苷标记的膀胱组织中蛋白酶诱导的细胞刺激的扫描电子显微镜研究。通过X射线分析证明了各个细胞核上银浓度的差异，并获得了定量数据，显示在蛋白酶处理的培养物中3H-胸腺嘧啶核苷掺入率增加。初步证据表明，X射线微量分析可为放射自显影提供一种潜在有用的定量方法。
