Fry D L, Tousimis A J, Talbot T L, Lewis S J
Am J Physiol. 1980 Mar;238(3):H414-22. doi: 10.1152/ajpheart.1980.238.3.H414.
The developed silver in specially prepared photographic films (PF) and autoradiographs (AR) of radiolabeled arterial tissue was quantified by direct grain counting (GC), microdensitometry (OD), and by electron probe X-ray microanalysis (EPA). The EPA data was proportional to the OD data with a very small variance. The GC data increased with the EPA data, but showed a large variance. The EPA signal was shown 1) to be reproducible even after multiple traverses across the specimen, 2) to be directly proportional to the electron beam current and emulsion silver concentration, and 3) to be insensitive to a) beam size, b) current density, c) energy above 17 keV, or d) nonuniformities in the thickness of the conductive coating on the specimen.
通过直接颗粒计数（GC）、显微密度测定法（OD）以及电子探针X射线微分析（EPA）对特殊制备的放射性标记动脉组织的摄影胶片（PF）和放射自显影片（AR）中显影的银进行定量分析。EPA数据与OD数据成比例，方差非常小。GC数据随EPA数据增加，但显示出较大的方差。EPA信号表明：1）即使在多次穿过标本后仍具有可重复性；2）与电子束电流和乳剂银浓度成正比；3）对以下因素不敏感：a）束斑大小；b）电流密度；c）高于17keV的能量；d）标本上导电涂层厚度的不均匀性。
