Pruessner Jens C, Köhler Stefan, Crane Joelle, Pruessner Marita, Lord Catherine, Byrne Andrea, Kabani Noor, Collins D Louis, Evans Alan C
McConnell Brain Imaging Center, Montreal Neurological Institute, Douglas Hospital Research Center, McGill University, Montreal, Quebec, Canada.
Cereb Cortex. 2002 Dec;12(12):1342-53. doi: 10.1093/cercor/12.12.1342.
Researchers in clinical and basic neuroscience frequently target structures of the human medial temporal lobe (MTL) for volumetric analysis with magnetic resonance imaging (MRI). In neurodegenerative diseases, a precise volumetric analysis of MTL structures can assist in differential diagnosis and can be used in guiding early treatment. Also, in functional neuroimaging, exact localization is crucial for the correct interpretation of focal MTL activations with respect to specific memory functions. In presently available protocols, precise and consistent volumetric analysis of MTL structures is compromised in numerous ways. Most importantly, in order to cover all structures of the MTL, the researcher is presently forced to combine independently developed segmentation protocols for different structures from different laboratories. This approach limits anatomical precision because these protocols are based on different anatomical guidelines and descriptions that cannot easily be integrated. The segmentation approach presented in this paper was designed to address this issue by presenting segmentation guidelines for all major structures of the parahippocampal gyrus (PHG). It was developed directly to complement a volumetric protocol for hippocampus and amygdala (Pruessner et al., 2000, Cereb Cortex 10:433-442), thus allowing volumetric assessment of all major MTL structures in an integrated and consistent manner. Furthermore, it takes into consideration the neuroanatomical appearance of the collateral sulcus by presenting a method to correct the volumes of the surrounding cortices for the variability of this sulcus. The protocol was validated using MR images of 40 healthy normal control subjects (20 men and 20 women, age range 18-42 years). Intra- and interrater coefficients are presented, together with mean values for the volumes of all PHG structures, correlations with age and sex, and tests for hemispheric differences.
临床和基础神经科学领域的研究人员经常将人类内侧颞叶（MTL）的结构作为磁共振成像（MRI）体积分析的目标。在神经退行性疾病中，对MTL结构进行精确的体积分析有助于鉴别诊断，并可用于指导早期治疗。此外，在功能神经成像中，精确的定位对于正确解释MTL特定区域与特定记忆功能相关的激活情况至关重要。在目前可用的方案中，MTL结构的精确和一致的体积分析在许多方面受到影响。最重要的是，为了涵盖MTL的所有结构，研究人员目前不得不将来自不同实验室针对不同结构独立开发的分割方案结合起来。这种方法限制了解剖学精度，因为这些方案基于不同的解剖学指南和描述，难以轻易整合。本文提出的分割方法旨在通过为海马旁回（PHG）的所有主要结构提供分割指南来解决这一问题。它是直接为补充海马体和杏仁核的体积测量方案而开发的（Pruessner等人，2000年，《大脑皮层》10:433 - 442），从而能够以综合和一致的方式对所有主要的MTL结构进行体积评估。此外，它通过提出一种方法来校正周围皮质的体积，以考虑侧副沟的神经解剖学外观变化。该方案使用40名健康正常对照受试者（20名男性和20名女性，年龄范围18 - 42岁）的MR图像进行了验证。给出了评分者内和评分者间系数，以及所有PHG结构体积的平均值、与年龄和性别的相关性，以及半球差异测试结果。
