Dullaghan Edith M, Brooks Patricia C, Davis Elaine O
Division of Mycobacterial Research, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK1.
Microbiology (Reading). 2002 Nov;148(Pt 11):3609-3615. doi: 10.1099/00221287-148-11-3609.
Four potential binding sites for LexA were identified upstream of the Mycobacterium tuberculosis lexA gene. A mutational analysis of these sites in a lexA-lacZ reporter construct revealed that only one of these SOS boxes was required for DNA-damage-mediated regulation of lexA expression. A novel DNA-damage-inducible gene, Rv2719c, was identified that was divergently transcribed relative to lexA; the other three SOS boxes were found to be involved in regulating expression of this novel mycobacterial-specific gene. The SOS boxes lay in the respective promoter regions of the genes that they regulated.
在结核分枝杆菌lexA基因上游鉴定出4个LexA潜在结合位点。对lexA - lacZ报告基因构建体中这些位点进行突变分析表明，DNA损伤介导的lexA表达调控仅需要其中一个SOS框。鉴定出一个新的DNA损伤诱导基因Rv2719c，它与lexA呈反向转录；发现其他三个SOS框参与调控这个新的分枝杆菌特异性基因的表达。这些SOS框位于它们所调控基因的各自启动子区域。
