Stöckbauer P
Ustav hematologie a krevní transfuze, Praha.
Cas Lek Cesk. 2002 Sep 22;141 Suppl:13-7.
Hematopoietic cell lines phenotypically and genotypically corresponding to original patient's leukemic or lymphoma cells are the powerful tools for studies of many aspects of malignant process and also for studies of normal and malignant hematopoesis. Until recently, permanent cell lines were derived from nearly all types of hematopoietic malignancies--leukemias and lymphomas. Availability of leukemia and lymphoma cell lines enabled the development of hybridoma technology for production of monoclonal antibodies and also discovery of the first human retroviruses HTLV-1,2 and HIV. Last but not least, leukemic cell lines are also used as standards for molecular diagnostics of leukemias and lymphomas. Leukemic and lymphoma cell lines have fully proved and extended their importance for modeling of malignant process ex vivo.
在表型和基因型上与原始患者白血病或淋巴瘤细胞相对应的造血细胞系，是研究恶性过程诸多方面以及正常和恶性造血的有力工具。直到最近，几乎所有类型的造血恶性肿瘤——白血病和淋巴瘤，都已获得了永久细胞系。白血病和淋巴瘤细胞系的可得性推动了用于生产单克隆抗体的杂交瘤技术的发展，还促成了首批人类逆转录病毒HTLV-1、HTLV-2和HIV的发现。最后但同样重要的是，白血病细胞系也被用作白血病和淋巴瘤分子诊断的标准。白血病和淋巴瘤细胞系已充分证明并扩展了它们在体外模拟恶性过程方面的重要性。
