Van Hoogmoed Linda M, Snyder Jack R, Harmon Faye A
Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
Am J Vet Res. 2002 Nov;63(11):1496-500. doi: 10.2460/ajvr.2002.63.1496.
To evaluate the effect of 2 cyclooxygenase (COX)-2 inhibitors on contractile activity of the circular smooth muscle layer of the equine dorsal and ventral colon.
Samples of the dorsal and ventral colon obtained from 10 healthy horses.
Full-thickness tissue samples were collected from the dorsal colon in the area of the diaphragmatic flexure and the ventral colon in the area of the sternal flexure. Samples were cut into strips oriented along the fibers of the circular muscle layer and mounted in a tissue bath system for determination of contractile strength. Incremental amounts of etodolac, nabumetone, and indomethacin were added, and contractile activity was recorded.
Response of the dorsal and ventral colon to nonsteroidal anti-inflammatory drugs (NSAIDs) was variable. Indomethacin induced the greatest reduction in contractile activity, followed by nabumetone. For etodolac, the difference from baseline values was only significantly reduced at the highest concentration used (1 X 10(5)M) for the ventral colon.
The NSAIDs that are designed to target the COX-2 isoform appeared to have variable effects on the contractile activity of the equine dorsal and ventral colon. Etodolac appeared to have the least effect on contractile activity, compared with the effects attributable to nabumetone, and would potentially have the fewest adverse effects relative to motility of the dorsal and ventral colon.
评估2种环氧化酶（COX）-2抑制剂对马背侧和腹侧结肠环形平滑肌层收缩活性的影响。
从10匹健康马获取背侧和腹侧结肠样本。
从膈曲区域的背侧结肠和胸骨曲区域的腹侧结肠采集全层组织样本。将样本切成沿环形肌层纤维方向的条带，并安装在组织浴系统中以测定收缩强度。添加递增剂量的依托度酸、萘丁美酮和吲哚美辛，并记录收缩活性。
背侧和腹侧结肠对非甾体抗炎药（NSAIDs）的反应各不相同。吲哚美辛引起的收缩活性降低最大，其次是萘丁美酮。对于依托度酸，仅在腹侧结肠使用的最高浓度（1×10⁵M）下，与基线值的差异才显著降低。
旨在靶向COX-2同工型的NSAIDs对马背侧和腹侧结肠的收缩活性似乎有不同影响。与萘丁美酮相比，依托度酸对收缩活性的影响似乎最小，并且相对于背侧和腹侧结肠的运动性，其潜在的不良反应可能最少。
