DeBoer Douglas J, Moriello Karen A, Blum Jenifer L, Volk Lynn M, Bredahl Lars K
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
Am J Vet Res. 2002 Nov;63(11):1532-7. doi: 10.2460/ajvr.2002.63.1532.
To determine antidermatophyte immunologic effects of an experimental combined live-inactivated dermatophytosis vaccine (CLIDV) and a commercial inactivated dermatophytosis vaccine (IDV) in cats and to evaluate adverse effects associated with administration of these vaccines.
20 healthy juvenile domestic shorthair cats.
Cats were injected with 2 doses of CLIDV at the standard dosage or 1 dose of CLIDV at 10 times the standard dosage; IDV was administered at the manufacturer-recommended dosage. Cats were observed for illness and reactions at inoculation sites. Periodically, samples were obtained for fungal culture, lymphocyte blastogenesis test (LBT) as an indicator of cell-mediated immunity against dermatophyte antigens, and antidermatophyte IgG titers. Following vaccination, cats were challenge-exposed by topical application of Microsporum canis macroconidia and examined weekly for clinical signs of dermatophytosis.
of 10 cats given CLIDV developed focal crusts at the injection site that resolved without treatment; these were areas of dermatophyte infection with the vaccine strain. Antidermatophyte IgG titers increased significantly with all vaccination protocols. Cellular immunity against M canis increased slightly and variably during the vaccination period and did not differ significantly between vaccinated and control cats. All cats developed dermatophyte infection after challenge exposure. Vaccination with CLIDV or IDV was associated with slightly reduced severity of initial infection.
Noculation with IDV or CLIDV did not provide prophylactic immunity against topical challenge exposure with M canis. Inoculation with either vaccine did not provide a more rapid cure of an established infection.
确定实验性联合活-灭活皮肤癣菌病疫苗（CLIDV）和市售灭活皮肤癣菌病疫苗（IDV）对猫的抗皮肤癣菌免疫作用，并评估与这些疫苗接种相关的不良反应。
20只健康的幼年家养短毛猫。
猫以标准剂量注射2剂CLIDV或1剂10倍标准剂量的CLIDV；IDV按制造商推荐剂量给药。观察猫的疾病情况及接种部位的反应。定期采集样本进行真菌培养、淋巴细胞增殖试验（LBT）作为细胞介导的针对皮肤癣菌抗原免疫的指标，以及抗皮肤癣菌IgG滴度检测。接种疫苗后，通过局部应用犬小孢子菌大分生孢子对猫进行激发暴露，并每周检查皮肤癣菌病的临床症状。
接受CLIDV的10只猫在注射部位出现局灶性结痂，未经治疗自行消退；这些是疫苗株皮肤癣菌感染区域。所有接种方案的抗皮肤癣菌IgG滴度均显著升高。在接种期间，针对犬小孢子菌的细胞免疫略有增加且变化不定，接种疫苗的猫与对照猫之间无显著差异。所有猫在激发暴露后均发生皮肤癣菌感染。接种CLIDV或IDV与初始感染的严重程度略有降低相关。
接种IDV或CLIDV不能提供针对犬小孢子菌局部激发暴露的预防性免疫。接种任何一种疫苗都不能使已建立的感染更快治愈。
