DeBoer D J, Moriello K A
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
Res Vet Sci. 1995 Sep;59(2):110-3. doi: 10.1016/0034-5288(95)90042-x.
A laboratory-prepared killed Microsporum canis cell-wall vaccine was evaluated under conditions simulating an accidental infection of a cattery, by inoculating eight- to nine-week-old cats with the vaccine or with a placebo control. The vaccinated cats developed high titres of anti-dermatophyte IgG as measured by an ELISA, and a small cell-mediated response against M canis as measured by a lymphocyte blastogenesis assay, using a whole fungus extract. After being inoculated the cats were challenged by the introduction of an infected cat into the same room. All the vaccinated and control cats became culture-positive for M canis within four weeks of the introduction of the infected cat. Four of the six control cats and all the vaccinated cats developed lesions consistent with dermatophytosis within 16 weeks after exposure to the infected cat.
一种实验室制备的犬小孢子菌细胞壁灭活疫苗，在模拟猫舍意外感染的条件下进行了评估，给8至9周龄的猫接种该疫苗或安慰剂对照。通过酶联免疫吸附测定（ELISA）检测，接种疫苗的猫产生了高滴度的抗皮肤癣菌IgG，并且使用全真菌提取物通过淋巴细胞增殖试验检测，对犬小孢子菌产生了微弱的细胞介导反应。接种后，将一只感染的猫引入同一房间对这些猫进行攻毒。在引入感染猫后的四周内，所有接种疫苗和对照的猫犬小孢子菌培养均呈阳性。在接触感染猫后的16周内，六只对照猫中的四只以及所有接种疫苗的猫都出现了与皮肤癣菌病相符的病变。
