Buluç Mesut, Gürdal Hakan, Melli Mehmet
Department of Pharmacology and Clinical Pharmacology, Medical Faculty of Ankara University, Turkey.
Prostaglandins Other Lipid Mediat. 2002 Sep;70(1-2):227-39. doi: 10.1016/s0090-6980(02)00112-0.
Effects of misoprostol, a synthetic prostaglandin E1 (PGE1) analogue, on cyclooxygenase-2 (COX-2) protein level and exudate prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) level were investigated in acute carrageenan-induced air pouch inflammation in rats. Treatment with misoprostol (12.5, 25, and 50 microg/kg) has been started in separated groups, 30 min and 2 days before carrageenan injection and it was given twice a day (total of five doses) by orogastric route. Indomethacin, in doses of 0.5 and 5 mg/kg, and specific COX-2 inhibitor SC-58236, in doses of 5, 10, and 20 mg/kg were given 1 h before carrageenan injection by the orogastric route. Misoprostol increased the levels of PGE2 and COX-2 protein at all doses applied. Despite indomethacin and SC-58236 increased the level of COX-2 protein when they used alone, these drugs partially inhibited misoprostol-induced increase in the level of COX-2 protein. Partial inhibition of misoprostol-induced increase in the level of COX-2 protein by indomethacin or SC-58236 may indicate the modulatory roles of endogenous prostaglandins (PGs, especially, PGE2) on the COX-2 expression.
在急性角叉菜胶诱导的大鼠气囊肿炎症模型中，研究了合成前列腺素E1（PGE1）类似物米索前列醇对环氧合酶-2（COX-2）蛋白水平以及渗出液中前列腺素E2（PGE2）和血栓素B2（TXB2）水平的影响。在角叉菜胶注射前30分钟和2天，分别对不同组大鼠开始用米索前列醇（12.5、25和50微克/千克）进行处理，通过灌胃途径给药，每天两次（共五剂）。在角叉菜胶注射前1小时，通过灌胃途径给予剂量为0.5和5毫克/千克的吲哚美辛以及剂量为5、10和20毫克/千克的特异性COX-2抑制剂SC-58236。所有应用剂量的米索前列醇均增加了PGE2和COX-2蛋白的水平。尽管吲哚美辛和SC-58236单独使用时会增加COX-2蛋白水平，但这些药物部分抑制了米索前列醇诱导的COX-2蛋白水平升高。吲哚美辛或SC-58236对米索前列醇诱导的COX-2蛋白水平升高的部分抑制可能表明内源性前列腺素（PGs，尤其是PGE2）对COX-2表达具有调节作用。
