Berenguer-Navarro V, Giner-Galván R M, Grané-Teruel N, Arrazola-Paternina G
Departamento de Tecnología de Alimentos, Universidad de Córdoba, Colombia.
J Agric Food Chem. 2002 Nov 20;50(24):6960-3. doi: 10.1021/jf0256081.
The determination of cyanogenic compounds in plants is often performed by HPLC. However, in this analysis, interferences due to compounds in the matrix, such as tannins and other pigments, are encountered, especially in roots and leaves. A new method is proposed for determining the cyanogenic glycosides amygdalin (D-mandelonitrile beta-D-gentiobioside) and prunasin (D-mandelonitrile beta-D-glucoside) in almond tree tissues, using poly(vinylpyrrolidone) or active carbon as scavengers for extracting cyanogenic compounds from roots or leaves, respectively. A new chromatographic approach for conducting the analysis is also discussed herein. The advantages of a Hypercarb column for the analysis of prunasin in roots are shown. The correlation coefficient with a reference method is high (>0.99), and statistical tests prove that the two methods are equivalent. In addition, the results provide evidence that prunasin is the only cyanoglycoside present in almond tree roots.
植物中氰化物的测定通常采用高效液相色谱法（HPLC）。然而，在该分析中，会遇到基质中化合物（如单宁和其他色素）产生的干扰，尤其是在根和叶中。本文提出了一种新方法，用于测定杏仁树组织中的氰苷苦杏仁苷（D-扁桃腈β-D-龙胆二糖苷）和樱叶苷（D-扁桃腈β-D-葡萄糖苷），分别使用聚乙烯吡咯烷酮或活性炭作为清除剂从根或叶中提取氰化物。本文还讨论了一种用于进行该分析的新色谱方法。展示了Hypercarb柱用于分析根中樱叶苷的优势。与参考方法的相关系数很高（>0.99），并且统计检验证明这两种方法等效。此外，结果表明樱叶苷是杏仁树根中唯一存在的氰苷。
