佐菲菌素对炭疽菌和葡萄孢菌抗真菌活性的pH调节

pH modulation of zopfiellin antifungal activity to Colletotrichum and Botrytis.

作者信息

Futagawa Mitsugu, Rimando Agnes M, Tellez Mario R, Wedge David E

机构信息

Shiraoka Research Station of Biological Science, Nissan Chemical Industries, Ltd., 1470 Shiraoka, Minamisaitama, Saitama 349-0294, Japan.

出版信息

J Agric Food Chem. 2002 Nov 20;50(24):7007-12. doi: 10.1021/jf025720z.

DOI:10.1021/jf025720z

PMID:12428951

Abstract

Zopfiellin, a novel cyclooctanoid natural product isolated from Zopfiella curvata No. 37-3, was evaluated in a 96-well microtiter assay for fungicidal activity against Botrytis cinerea, Colletotrichum acutatum, Colletotrichum fragariae, Colletotrichum gloeosporioides, and Fusarium oxysporum. Zopfiellin exhibited pH-dependent activity, with the most mycelial growth inhibition demonstrated at pH 5.0. Mass spectrometry and nuclear magnetic resonance spectroscopy studies indicated that zopfiellin undergoes structural changes with changes in pH. At pH 5.0, zopfiellin showed the greatest activity against B. cinerea (IC(80) = 10 microM), C. gloeosporioides (IC(80) = 10 microM), and C. fragariae (IC(80) = 10 microM) and intermediate activity against C. acutatum (IC(80) = 30 microM), and was not active against F. oxysporum (IC(80) > 100 microM).

摘要

佐菲菌素是从弯曲佐菲酵母37-3号菌株中分离出的一种新型环辛类天然产物，在96孔微量滴定试验中对灰葡萄孢、尖孢炭疽菌、草莓炭疽菌、胶孢炭疽菌和尖孢镰刀菌进行了杀真菌活性评估。佐菲菌素表现出pH依赖性活性，在pH 5.0时对菌丝生长的抑制作用最强。质谱和核磁共振光谱研究表明，佐菲菌素的结构会随pH值变化而改变。在pH 5.0时，佐菲菌素对灰葡萄孢（IC80 = 10微摩尔）、胶孢炭疽菌（IC80 = 10微摩尔）和草莓炭疽菌（IC80 = 10微摩尔）表现出最大活性，对尖孢炭疽菌表现出中等活性（IC80 = 30微摩尔），而对尖孢镰刀菌无活性（IC80 > 100微摩尔）。