Department of Medicinal Chemistry, University of Mississippi, University, Mississippi 38677, USA.
J Agric Food Chem. 2013 May 15;61(19):4551-5. doi: 10.1021/jf400212y. Epub 2013 May 7.
Bioassay-guided fractionation of Cladosporium cladosporioides (Fresen.) de Vries extracts led to the isolation of four compounds, including cladosporin, 1; isocladosporin, 2; 5'-hydroxyasperentin, 3; and cladosporin-8-methyl ether, 4. An additional compound, 5',6-diacetylcladosporin, 5, was synthesized by acetylation of compound 3. Compounds 1-5 were evaluated for antifungal activity against plant pathogens. Phomopsis viticola was the most sensitive fungus to the tested compounds. At 30 μM, compound 1 exhibited 92.7, 90.1, 95.4, and 79.9% growth inhibition against Colletotrichum acutatum , Colletotrichum fragariae , Colletotrichum gloeosporioides , and P. viticola, respectively. Compound 2 showed 50.4, 60.2, and 83.0% growth inhibition at 30 μM against Co. fragariae, Co. gloeosporioides, and P. viticola, respectively. Compounds 3 and 4 were isolated for the first time from Cl. cladosporioides. Moreover, the identification of essential structural features of the cladosporin nuclei has also been evaluated. These structures provide new templates for the potential treatment and management of plant diseases.
生物测定指导的枝孢属（Fresen.）de Vries提取物的分离导致了四种化合物的分离，包括枝孢菌素，1；异枝孢菌素，2；5'-羟基asperentin，3；和枝孢菌素-8-甲醚，4。通过化合物 3 的乙酰化合成了另一种化合物，5',6-二乙酰枝孢菌素，5。化合物 1-5 被评估了对植物病原体的抗真菌活性。葡萄座腔菌是对测试化合物最敏感的真菌。在 30 μM 时，化合物 1 对炭疽菌、草莓炭疽菌、胶孢炭疽菌和葡萄座腔菌的生长抑制率分别为 92.7%、90.1%、95.4%和 79.9%。化合物 2 在 30 μM 时对草莓炭疽菌、胶孢炭疽菌和葡萄座腔菌的生长抑制率分别为 50.4%、60.2%和 83.0%。化合物 3 和 4 是首次从枝孢属中分离出来的。此外，还评估了枝孢菌素核的必需结构特征的鉴定。这些结构为植物疾病的潜在治疗和管理提供了新的模板。
