Taylor P V, Hancock K W
Immunology. 1975 May;28(5):973-82.
The interaction between cultured human trophoblast cells and materanl lymphocytes was used as an in vitro model to investigate trophoblast antigenicity. Cytotoxic effects in the trophoblast monolayer were apparent after 72 hr incubation and depended on the presence of non-lymphoid cell types in addition to lymphocytes. Lysis of trophoblast was preceded by blast cell formation and apparently involved close contact between the maternal cells and the trophoblast cells. The nature of the cytotoxic reaction suggested the presence of histocompatibility factors on trypsinized trophoblast cells. This manifestation could be due to removal of fibrinoid or enhancing antibody from the cultured cells. The ability of trypsinized trophoblast cells to synthesize mucoprotein was investigated by the Hale colloidal iron test and found to be unimpaired. The effect of maternal serum on the recognition of human trophoblast antigens by maternal lymphocytes was used as an in vitro model to investigate the occurrence of enhancing antibody in maternal serum. The cytotoxic effects of maternal lymphocytes on trophoblast were completely prevented by the presence of maternal serum, this protective effect being reduced significantly by removal of IgG from the maternal serum. A slight protective effect of allogeneic pregnancy serum was also observed. It is suggested that these findings support a role for immunological enhancement in maintaining the foetal allograft.
将培养的人滋养层细胞与母体淋巴细胞之间的相互作用作为体外模型，以研究滋养层抗原性。滋养层单层细胞在孵育72小时后出现细胞毒性作用，并且除淋巴细胞外还取决于非淋巴细胞类型的存在。滋养层细胞溶解之前有母细胞形成，并且显然涉及母体细胞与滋养层细胞之间的密切接触。细胞毒性反应的性质表明胰蛋白酶处理过的滋养层细胞上存在组织相容性因子。这种表现可能是由于从培养细胞中去除了类纤维蛋白或增强抗体所致。通过黑尔胶体铁试验研究了胰蛋白酶处理过的滋养层细胞合成粘蛋白的能力，发现其未受损害。将母体血清对母体淋巴细胞识别人类滋养层抗原的影响作为体外模型，以研究母体血清中增强抗体的存在情况。母体血清的存在完全阻止了母体淋巴细胞对滋养层细胞的细胞毒性作用，从母体血清中去除IgG后，这种保护作用显著降低。还观察到同种异体妊娠血清有轻微的保护作用。有人提出，这些发现支持免疫增强在维持胎儿同种异体移植中的作用。
