Harbour-McMenamin D, Smith E M, Blalock J E
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6834-8. doi: 10.1073/pnas.83.18.6834.
Maternal immunologic recognition of the blastocyst as well as the hormone chorionic gonadotropin (CG) has been shown to be a requirement for successful implantation of the blastocyst and maintenance of the fetal graft. On the basis of these observations, we tested whether lymphocytes could produce chorionic gonadotropic-like hormones during a mixed lymphocyte reaction (MLR). Here we described the production of immunoreactive chorionic gonadotropin (ir-CG) as a result of an allogeneic stimulus but not other mitogenic stimuli. The kinetics of production, as monitored by immunofluorescence with antibody specific to the beta chain of the human CG (hCG) molecule, paralleled the blastogenic response. Gel filtration of the de novo-synthesized ir-CG showed the molecular weight of the intact molecule to be approximately 58,000. This molecule bound to a concanavalin A affinity column and also dissociated into two subunits of approximate molecular weights 32,000 and 18,000. These molecular weights are consistent with reported molecular weights for glycosylated intact hCG, as well as the beta and alpha chains of hCG, respectively. The material from the MLR but not the control cultures elicited significant testosterone production from Leydig cells, and this activity could be blocked by antiserum to hCG. The results described here, together with the fact that the blastocyst is implanted in a lymphocyte infiltrate, lend support to our postulate that lymphocyte-derived CG enhances successful implantation of the allogeneic fetus, thus providing a mechanism for selection of genetic diversity.
母体对胚泡以及激素绒毛膜促性腺激素（CG）的免疫识别已被证明是胚泡成功着床和维持胎儿移植物所必需的。基于这些观察结果，我们测试了淋巴细胞在混合淋巴细胞反应（MLR）过程中是否能产生绒毛膜促性腺激素样激素。在此我们描述了由于同种异体刺激而非其他促有丝分裂刺激导致的免疫反应性绒毛膜促性腺激素（ir-CG）的产生。通过用人绒毛膜促性腺激素（hCG）分子β链特异性抗体进行免疫荧光监测，其产生动力学与细胞增殖反应平行。对新合成的ir-CG进行凝胶过滤显示完整分子的分子量约为58,000。该分子能结合到伴刀豆球蛋白A亲和柱上，并且还可解离成分子量约为32,000和18,000的两个亚基。这些分子量分别与报道的糖基化完整hCG以及hCG的β链和α链的分子量一致。来自MLR的物质而非对照培养物能显著刺激睾丸间质细胞产生睾酮，并且这种活性可被hCG抗血清阻断。此处描述的结果，以及胚泡植入淋巴细胞浸润区域这一事实，支持了我们的假设，即淋巴细胞衍生的CG可增强同种异体胎儿的成功着床，从而为遗传多样性的选择提供了一种机制。
