Mechanic Steven A, Maurer Joan L, Igoe Mark J, Kavitsky Donna M, Nance Sandra T
Department of Pathology and Laboratory Medicine, Temple University Hospital, Philadelphia, Pennsylvania, USA.
Transfusion. 2002 Sep;42(9):1180-3. doi: 10.1046/j.1537-2995.2002.00187.x.
An anti-Vel, nearly missed in antibody identification studies, and the effect of a commercially available rabbit RBC stroma (RESt, Immucor) adsorptions on eight anti-Vel sera are reported. Anti-Vel is an antibody to an antigen of high prevalence.
A 48-year-old woman with chronic vaginal bleeding presented with a Hct of 14.7 percent. The transfusion service was not informed of her history of anti-Vel when she was transferred from another institution. Studies performed on an emergency request for transfusion were interpreted as a cold autoantibody as adsorption with a commercial source of RESt eliminated the reactivity. Stored anti-Vel sera were tested by titration studies before and after adsorption with commercial RESt.
Serum from the index case did not react after adsorption with RESt at the transfusion service. Studies with the stored anti-Vel indicated antibody adsorption with four of four samples at immediate spin (IS) and room temperature (RT) phases, four of eight samples at 37 degrees C in albumin (ALB) phase, and four of eight samples at ALB-IgG-AGT phase. Variations in antibody reactivity were observed in the samples tested, but RESt adsorption diminished antibody reactivity in most samples. All eight stored sera demonstrated some reactivity in at least one phase after adsorption with RESt.
Anti-Vel was completely or partially adsorbed by RESt. Caution should be used when interpreting cold agglutinins with this method. The manufacturer warns that uncommon alloantibodies may be adsorbed.
报道了一种在抗体鉴定研究中险些漏检的抗-Vel抗体，以及市售兔红细胞基质（RESt，Immucor）吸附对8份抗-Vel血清的影响。抗-Vel是一种针对高流行率抗原的抗体。
一名48岁慢性阴道出血女性，血细胞比容为14.7%。当她从另一家机构转来时，输血科未被告知她有抗-Vel抗体史。因紧急输血请求所做的检测被解释为冷自身抗体，因为用市售RESt吸附后消除了反应性。在用市售RESt吸附前后，通过滴定研究对储存的抗-Vel血清进行检测。
在输血科，索引病例的血清用RESt吸附后无反应。对储存的抗-Vel血清的研究表明，在立即离心（IS）和室温（RT）阶段，4份样本中有4份抗体被吸附；在37℃白蛋白（ALB）阶段，8份样本中有4份抗体被吸附；在ALB-IgG-AGT阶段，8份样本中有4份抗体被吸附。在所检测的样本中观察到抗体反应性存在差异，但RESt吸附使大多数样本中的抗体反应性降低。所有8份储存血清在用RESt吸附后至少在一个阶段表现出一定反应性。
抗-Vel被RESt完全或部分吸附。用这种方法解释冷凝集素时应谨慎。制造商警告说，罕见的同种抗体可能会被吸附。
