Jiménez Marco M T, Hernández Maraver D, Rodríguez de la Rua A, de la Cámara Mendizábal C, Canales Albendea M, Jiménez Yuste V, Hernández Navarro F
Servicio de Hematología y Hemoterapia, Hospital Universitario La Paz, Madrid.
Sangre (Barc). 1998 Feb;43(1):21-4.
The polyethylene glycol antiglobulin test has been found to enhance the reactivity of most alloantibodies.
To investigate the utility of polyethylene glycol antiglobulin test for detection and identification of red blood cell antibodies, a comparison study of polyethylene glycol (PEG), a low-ionic-strength additive solution (LISS) and bovine serum albumin (BSA) was conducted. The sera of 47 different patients with positive antibody screening test by the LISS method, were tested in parallel with reagent antibody-detection cells using PEG, LISS and BSA.
In the sera of 47 patients, 57 antibodies were detected. We identified 39 antibodies by the three methods. Twelve antibodies reacted by the BSA method and the LISS method but did not react with the PEG method (8 anti-I, 1 anti-P1, 1 anti-Lea(a), and two antibodies missed by the PEG method because they did not react with anti-IgG: 1 anti-M and 1 anti-K). Three antibodies reacted only with the LISS method (3 anti-I). Four clinically significant antibodies were detected only by the PEG method (2 anti-Jka, 1 anti-Jkb, 1 anti-c). The serum from a patient with delayed hemolytic transfusion reaction and no antibody detectable by the LISS and the BSA methods was tested by the PEG method. We were able to detect an anti-Jka by PEG in the pretransfusion sample. In 24 (60%) of 40 samples with clinically significant antibodies, PEG antiglobulin reactions were stronger (total score 221) than LISS antiglobulin reactions (total score 170) and BSA antiglobulin reaction (total score 184); in 14 (35%) of 40 samples, they were identical, and in 2 (5%) agglutination in the PEG method was weaker.
In our experience, the polyethylene glycol antiglobulin test is more sensitive than LISS and BSA in detecting clinically significant antibodies and is an acceptable technique for routine compatibility test.
已发现聚乙二醇抗球蛋白试验可增强大多数同种抗体的反应性。
为研究聚乙二醇抗球蛋白试验在检测和鉴定红细胞抗体中的效用，对聚乙二醇（PEG）、低离子强度添加剂溶液（LISS）和牛血清白蛋白（BSA）进行了一项对比研究。对47例经LISS法抗体筛查试验呈阳性的不同患者的血清，使用PEG、LISS和BSA与试剂抗体检出细胞进行平行检测。
在47例患者的血清中，检测到57种抗体。我们通过三种方法鉴定出39种抗体。12种抗体通过BSA法和LISS法发生反应，但不与PEG法发生反应（8种抗-I、1种抗-P1、1种抗-Lea(a)，以及两种因不与抗-IgG发生反应而被PEG法漏检的抗体：1种抗-M和1种抗-K）。3种抗体仅与LISS法发生反应（3种抗-I）。4种具有临床意义的抗体仅通过PEG法检测到（2种抗-Jka、1种抗-Jkb、1种抗-c）。对一名发生迟发性溶血性输血反应且LISS法和BSA法均未检测到抗体的患者的血清进行PEG法检测。我们能够在输血前样本中通过PEG检测到一种抗-Jka。在40份具有临床意义抗体的样本中，24份（60%）的PEG抗球蛋白反应比LISS抗球蛋白反应（总分170）和BSA抗球蛋白反应（总分184）更强；在40份样本中的14份（35%）中，它们相同，在2份（5%）中PEG法的凝集较弱。
根据我们的经验，聚乙二醇抗球蛋白试验在检测具有临床意义的抗体方面比LISS和BSA更敏感，是一种可接受的常规相容性检测技术。
