Barboza de Martinez Yasmina, Ferrer Kenna, Salas Enrique Marquez
Unidad De Investigación en Ciencia y Tecnología De Alimentos, Facultad De Ciencias Veterinarias Universidad Del Zulia, Maracaibo, Venezuela.
J Food Prot. 2002 Nov;65(11):1780-3. doi: 10.4315/0362-028x-65.11.1780.
Changes in bacterial counts on beef carcasses at specific points during slaughter and fabrication were determined, and the effectiveness of nisin, lactic acid, and a combination of the lactic acid and nisin in reducing levels of microbiological contamination was assessed. Swab samples were obtained from the surfaces of randomly selected beef carcasses. Carcasses were swabbed from the neck, brisket, and renal site after skinning, splitting, and washing. Treatments involving lactic acid (1.5%), nisin (500 IU/ml), or a mixture of nisin and lactic acid were applied after the neck area was washed. A control group was not sprayed. Results indicated that the highest prevalence of aerobic plate counts (APCs), total coliforms, and Escherichia coli was found in the neck site after splitting, and the lowest level of microbial contamination was found after skinning. Washing with water did not significantly reduce the bacterial load. The largest reduction in APCs, total coliforms, and E. coli occurred on carcasses treated with a mixture of nisin and lactic acid. A mixture of nisin and lactic acid can be applied to beef carcasses through spray washing and can reduce bacterial populations by 2 log units.
测定了屠宰和加工过程中特定时间点牛肉胴体上细菌数量的变化，并评估了乳酸链球菌素、乳酸以及乳酸与乳酸链球菌素组合在降低微生物污染水平方面的有效性。从随机选择的牛肉胴体表面采集拭子样本。在剥皮、劈半和清洗后，从颈部、胸部和肾脏部位对胴体进行擦拭取样。在颈部区域清洗后，采用含1.5%乳酸、500 IU/ml乳酸链球菌素或乳酸链球菌素与乳酸混合物的处理方法。对照组不进行喷雾处理。结果表明，劈半后颈部部位的需氧平板计数（APC）、总大肠菌群和大肠杆菌的检出率最高，而剥皮后微生物污染水平最低。用水清洗并未显著降低细菌载量。乳酸链球菌素与乳酸混合物处理的胴体上，APC、总大肠菌群和大肠杆菌的减少量最大。乳酸链球菌素与乳酸的混合物可通过喷雾清洗应用于牛肉胴体，可使细菌数量减少2个对数单位。
