Castillo A, Lucia L M, Mercado I, Acuff G R
Texas A&M University, Department of Animal Science, College Station 77843-2471, USA.
J Food Prot. 2001 May;64(5):738-40. doi: 10.4315/0362-028x-64.5.738.
The effectiveness of a lactic acid treatment consisting of spraying a 4% L-lactic acid solution (55 degrees C at source) on chilled beef carcasses to reduce bacterial populations was tested in a commercial slaughter environment. All carcasses had been treated with a proprietary decontamination treatment composed of a hot water spray followed by a lactic acid spray prior to chilling. Bacterial groups used to indicate reductions included aerobic plate count (APC), total coliform count, and Escherichia coli count, and samples were examined from the brisket, the clod, and the neck regions of 40 untreated and 40 treated carcass sides. Depending on the carcass surface region, APCs were reduced by 3.0 to 3.3 log cycles. Log coliform and E. coli counts were consistently reduced to undetectable levels. The small reductions observed for coliforms are attributable to counts on untreated carcasses already being near the lower detection limit of the counting method. The percentage of samples with detectable numbers of coliforms (positive samples) on untreated carcasses ranged from 52.5 to 92.5%, while 0.0% of the samples collected from treated carcasses contained detectable coliforms. Percent E. coli-positive samples ranged from 7.5 to 30.0% on untreated carcasses and 0.0% after treatment of carcass sides. These results indicate that a hot lactic acid spray with increased concentration and time of application may be effectively implemented for an additional decontamination treatment of chilled beef carcasses prior to fabrication.
在商业屠宰环境中，测试了一种乳酸处理方法的有效性，该方法是将4%的L-乳酸溶液（源温度为55摄氏度）喷洒在冷却牛肉胴体上以减少细菌数量。所有胴体在冷却前都经过了一种专有的去污处理，包括热水喷淋，然后是乳酸喷淋。用于指示减少情况的细菌类别包括需氧平板计数（APC）、总大肠菌群计数和大肠杆菌计数，从40个未处理和40个处理过的胴体半边的胸部、肩部和颈部区域采集样本进行检测。根据胴体表面区域的不同，APC减少了3.0至3.3个对数周期。大肠菌群和大肠杆菌的对数计数始终降至检测不到的水平。大肠菌群的小幅减少归因于未处理胴体上的计数已接近计数方法的检测下限。未处理胴体上可检测到大肠菌群数量的样本百分比（阳性样本）在52.5%至92.5%之间，而从处理过的胴体采集的样本中0.0%含有可检测到的大肠菌群。未处理胴体上大肠杆菌阳性样本的百分比在7.5%至30.0%之间，胴体半边处理后为0.0%。这些结果表明，在分割前对冷却牛肉胴体进行额外的去污处理时，可以有效地采用浓度和施用时间增加的热乳酸喷淋方法。
