锁核酸酶：将锁核酸类型的单体掺入脱氧核酶中可显著增强RNA切割作用。

LNAzymes: incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage.

作者信息

Vester Birte, Lundberg Lars Bo, Sørensen Mads D, Babu B Ravindra, Douthwaite Stephen, Wengel Jesper

机构信息

The Nucleic Acid Center, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.

出版信息

J Am Chem Soc. 2002 Nov 20;124(46):13682-3. doi: 10.1021/ja0276220.

DOI:10.1021/ja0276220

PMID:12431091

Abstract

Incorporation of two alpha-L-LNA/LNA nucleotides into each of the two binding arms of a "10-23" DNAzyme has been accomplished and the RNA cleavage with these novel LNAzymes studied. In comparison with the unmodified DNAzyme, the LNAzymes show significantly improved cleavage of the phosphodiester backbone at the target nucleotide in a small RNA substrate (58n RNA) under single-turnover conditions. The LNAzymes show efficient multiple turnover. With the LNAzymes, efficient cleavage was accomplished also of a naturally occurring ribosomal RNA at a target site within a highly structured region. The reference DNAzyme was ineffective at cleaving the ribosomal RNA target.

摘要

已成功将两个α-L-LNA/LNA核苷酸掺入“10-23”脱氧核酶的两个结合臂中，并对这些新型LNA酶的RNA切割进行了研究。与未修饰的脱氧核酶相比，在单周转条件下，LNA酶在小RNA底物（58n RNA）中的靶核苷酸处对磷酸二酯主链的切割显著改善。LNA酶显示出高效的多周转。使用LNA酶，在高度结构化区域内的靶位点对天然存在的核糖体RNA也实现了有效切割。参考脱氧核酶在切割核糖体RNA靶标方面无效。