Priya K, Madhavan H N
L and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, India.
J Postgrad Med. 2002 Jul-Sep;48(3):176-8; discussion 190.
Since interpretation of results of enzyme linked immuno-sorbent assay (ELISA) for diagnosis of Cytomegalovirus (CMV) infection in India is difficult, its diagnostic value required evaluation.
To evaluate the diagnostic value of ELISA against polymerase chain reaction (PCR) in CMV disease.
Results of ELISA test for CMV antibodies in CMV-DNA PCR positive and negative patients and normal healthy blood donors were analysed.
Anti-CMV antibodies were assayed by ELISA on the sera of 26 CMV PCR positive and 21 PCR negative patients and 35 normal healthy blood donors.
Chi square and Fischer exact test were used for statistical analysis.
Anti-CMV antibodies (IgG or IgG and IgM) were present in 20 (76.9%) of 26 PCR positive and 13 (61.9%) of 21 PCR negative patients. ELISA was negative in six (23.1%) of 26 PCR positive patients. Of the 28 paediatric patients, ELISA was positive in 14 (73.7%) of 19 PCR positive and three (33.3%) of nine PCR negative patients showing a statistically significant difference (Chi square test, P value 0.038). Among the 19 patients having complications after organ transplant, ELISA showed anti-CMV antibodies in six (85.7%) of seven PCR positive and 11 (91.7%) of 12 PCR negative patients showing no significant difference. CMV-DNA was not detected in the buffy coat of 35 sero-positive blood donors.
ELISA has no diagnostic value in the detection of CMV activation although it may help in the differential diagnosis of CMV infection in the paediatric age group.
由于在印度酶联免疫吸附测定（ELISA）用于诊断巨细胞病毒（CMV）感染的结果解读困难，其诊断价值需要评估。
评估ELISA相对于聚合酶链反应（PCR）在CMV疾病中的诊断价值。
分析CMV - DNA PCR阳性和阴性患者以及正常健康献血者的CMV抗体ELISA检测结果。
采用ELISA法检测26例CMV PCR阳性患者、21例PCR阴性患者和35名正常健康献血者血清中的抗CMV抗体。
采用卡方检验和费舍尔精确检验进行统计分析。
26例PCR阳性患者中有20例（76.9%）存在抗CMV抗体（IgG或IgG和IgM），21例PCR阴性患者中有13例（61.9%）存在。26例PCR阳性患者中有6例（23.1%）ELISA结果为阴性。在28例儿科患者中，19例PCR阳性患者中有14例（73.7%）ELISA结果为阳性，9例PCR阴性患者中有3例（33.3%）ELISA结果为阳性，差异具有统计学意义（卡方检验，P值0.038）。在19例器官移植后出现并发症的患者中，7例PCR阳性患者中有6例（85.7%）ELISA显示存在抗CMV抗体，12例PCR阴性患者中有11例（91.7%）ELISA显示存在抗CMV抗体，差异无统计学意义。35名血清学阳性献血者的血沉棕黄层中未检测到CMV - DNA。
ELISA在检测CMV激活方面无诊断价值，尽管它可能有助于儿科年龄组CMV感染的鉴别诊断。
