Gandhoke I, Aggarwal R, Hussain S A, Pasha S T, Sethi P, Thakur S, Lal S, Khare S
Department of Microbiology, National Institute of Communicable Diseases, Directorate General of Health Services, 22 Shamnath Marg, Delhi, India.
Indian J Med Microbiol. 2009 Jul-Sep;27(3):222-5. doi: 10.4103/0255-0857.53204.
Samples from babies exhibiting clinical symptoms suggestive of congenital infection are referred regularly to NICD, New Delhi,, from Government Hospitals located in Delhi and a home for abandoned children (Palna), for the diagnosis of etiological agents like toxoplasma, rubella, CMV and herpes. Blood samples of mothers of most of the affected babies are also received.
Evaluation of rapid and accurate technique for the diagnosis of congenital CMV infection.
One hundred and twenty five blood samples suggestive of symptomatic congenital CMV infection were selected from samples received at NICD during the period June 2005-March 2007. A request to collect and send the urine samples of the selected babies was sent to the respective hospitals. Serum samples of the babies were tested for CMV-IgM antibodies using micro-capture ELISA. Mothers' serum samples were subjected to CMV-IgM and IgG class antibodies assay by commercial ELISA kits. DNA isolation and amplification was performed in urine samples and some of the serum samples using a commercial PCR kit for detection of HCMV. Blood and urine samples from 20 normal babies were included in the study.
Twenty Seven serum samples (21.6%) of infants, of the 125 tested, were positive for CMV-IgM antibodies. Twenty five samples (20%) showed amplification of CMV-DNA. All 25 samples positive for PCR were positive for CMV IgM antibodies. Sera of 73 mothers, out of 75 tested (97.3%), were positive for CMV IgG antibodies. However, none of them was positive for CMV IgM antibodies. Mothers of all 27 positive babies were positive for CMV-IgG antibodies. Serum and urine samples from 20 normal babies were negative for ELISA and PCR.
micro-capture ELISA technique was found to be more sensitive than PCR (92.6%) for detection of congenital CMV infection. ELISA is also rapid, less cumbersome and cost effective for diagnosis of CMV infection.
表现出先天性感染临床症状的婴儿样本定期从位于德里的政府医院以及一家弃儿院（帕尔纳）送往新德里的国家传染病中心（NICD），以诊断弓形虫、风疹、巨细胞病毒和疱疹等病原体。大多数受影响婴儿母亲的血液样本也会被收到。
评估诊断先天性巨细胞病毒感染的快速准确技术。
从2005年6月至2007年3月期间在NICD收到的样本中，选取125份提示有症状性先天性巨细胞病毒感染的血液样本。向各医院发出收集并送检所选婴儿尿液样本的请求。使用微捕获酶联免疫吸附测定法（ELISA）检测婴儿血清样本中的巨细胞病毒IgM抗体。母亲的血清样本通过商用ELISA试剂盒进行巨细胞病毒IgM和IgG类抗体检测。使用商用PCR试剂盒对尿液样本和部分血清样本进行DNA分离和扩增，以检测人巨细胞病毒（HCMV）。研究纳入了20名正常婴儿的血液和尿液样本。
在检测的125名婴儿中，27份血清样本（21.6%）的巨细胞病毒IgM抗体呈阳性。25份样本（20%）显示巨细胞病毒DNA扩增。所有25份PCR阳性样本的巨细胞病毒IgM抗体均为阳性。在检测的75名母亲中，73名（97.3%）的血清巨细胞病毒IgG抗体呈阳性。然而，她们中没有一人的巨细胞病毒IgM抗体呈阳性。所有27名阳性婴儿的母亲的巨细胞病毒IgG抗体均为阳性。20名正常婴儿的血清和尿液样本的ELISA和PCR检测均为阴性。
发现微捕获ELISA技术在检测先天性巨细胞病毒感染方面比PCR（92.6%）更敏感。ELISA在诊断巨细胞病毒感染方面也快速、操作简便且具有成本效益。
