反硝化产碱菌ITRC-4在有氧和无氧条件下对滴滴涕[1,1,1-三氯-2,2-双(4-氯苯基)乙烷]的代谢

Metabolism of DDT [1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane] by Alcaligenes denitrificans ITRC-4 under aerobic and anaerobic conditions.

作者信息

Ahuja Rajiv, Kumar Ashwani

机构信息

Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow 226 001, India.

出版信息

Curr Microbiol. 2003 Jan;46(1):65-9. doi: 10.1007/s00284-002-3819-1.

DOI:10.1007/s00284-002-3819-1

PMID:12432467

Abstract

An isolated bacterium, Alcaligenes denitrificans ITRC-4, metabolizes 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) under both aerobic and anaerobic conditions. The aerobic metabolism is inhibited by 38% and 47% in the presence of 1.0 g L(-1) of sodium acetate and sodium succinate, respectively, but remains uninhibited in the presence of 1.0 g L(-1) of glucose. Also, the metabolism is inhibited completely in the presence of biphenyl vapors, as well as 0.8 g L(-1) of 2,2'-bipyridyl. Under anaerobic conditions, DDT is metabolized into 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), which is further enhanced by 50% in the presence of 1.0 g L(-1) of glucose. Besides, the bacterium also metabolizes 4-chlorobenzoate, which is accompanied by the release of chloride ions.

摘要

一种分离出的细菌，反硝化产碱菌ITRC - 4，在有氧和无氧条件下均能代谢1,1,1 - 三氯 - 2,2 - 双（4 - 氯苯基）乙烷（滴滴涕）。在分别存在1.0 g L⁻¹ 乙酸钠和琥珀酸钠的情况下，有氧代谢分别受到38%和47%的抑制，但在存在1.0 g L⁻¹ 葡萄糖的情况下仍不受抑制。此外，在联苯蒸汽以及0.8 g L⁻¹ 2,2'-联吡啶存在时，代谢完全受到抑制。在无氧条件下，滴滴涕被代谢为1,1 - 二氯 - 2,2 - 双（4 - 氯苯基）乙烷（滴滴滴），在存在1.0 g L⁻¹ 葡萄糖的情况下，其代谢进一步增强50%。此外，该细菌还能代谢4 - 氯苯甲酸，同时伴有氯离子的释放。