Zatonsky George V, Kocharova Nina A, Veremeychenko Stanislav P, Zdorovenko Evelina L, Shashkov Alexander S, Zdorovenko Galina M, Knirel Yuriy A
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russian Federation.
Carbohydr Res. 2002 Nov 19;337(21-23):2365-70. doi: 10.1016/s0008-6215(02)00220-3.
The O-specific polysaccharide of P. fluorescens IMV 2366 was studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D gsCOSY, TOCSY, gsNOESY, H-detected 1H,(13)C gsHSQC, HMQC-TOCSY, and gsHMBC experiments. The polysaccharide contains L-rhamnose, 2-acetamido-2,6-dideoxy-D-galactose (D-FucNAc) and 3-acylamido-3,6-dideoxy-D-glucose (D-Qui3NAcyl, where Acyl is 3-hydroxy-2,3-dimethyl-5-oxoprolyl). The structure 1 of the polysaccharide was found to be similar to the structure 2 of a 6-deoxy-L-talose (L-6dTal)-containing O-specific polysaccharide of a non-classified P. fluorescens strain, 361, studied earlier [Khomenko, V. A.; Naberezhnykh, G. A.; Isakov, V. V.; Solov'eva, T. F.; Ovodov, Y. S.; Knirel, Y. A.; Vinogradov, E. V. Bioorg. Khim. 1986, 12, 1641-1648; Naberezhnykh, G. A.; Khomenko, V. A.; Isakov, V. V., El'kin, Y. N.; Solov'eva, T. F.; Ovodov, Y. S. Bioorg. Khim. 1987, 13, 1428-1429]. --> 2)-beta-D-Quip3NAcyl-(1 --> 3)-alpha-L-Rhap-(1 --> 3)-alpha-D-FucpNAc-(1 --> 1. --> 4)-beta-D-Quip3NAcyl-(1 --> 3)-alpha-L-6dTalp4Ac-(1 --> 3)-alpha-D-FucpNAc-(1 -->2.
通过糖分析、甲基化分析以及一维和二维核磁共振光谱（包括二维全相关谱、总相关谱、全核Overhauser效应谱、氢检测的异核单量子相干谱、异核多量子相干 - 总相关谱和全同核多键相关实验）对荧光假单胞菌IMV 2366的O - 特异性多糖进行了研究。该多糖含有L - 鼠李糖、2 - 乙酰氨基 - 2,6 - 二脱氧 - D - 半乳糖（D - FucNAc）和3 - 酰氨基 - 3,6 - 二脱氧 - D - 葡萄糖（D - Qui3NAcyl，其中酰基为3 - 羟基 - 2,3 - 二甲基 - 5 - 氧代脯氨酰基）。发现该多糖的结构1与先前研究的未分类荧光假单胞菌菌株361的含6 - 脱氧 - L - 塔罗糖（L - 6dTal）的O - 特异性多糖的结构2相似[霍缅科，V. A.；纳贝雷日尼克，G. A.；伊萨科夫，V. V.；索洛维耶娃，T. F.；奥沃多夫，Y. S.；克尼雷尔，Y. A.；维诺格拉多夫，E. V. 《生物有机化学》1986年，12卷，1641 - 1648页；纳贝雷日尼克，G. A.；霍缅科，V. A.；伊萨科夫，V. V.，叶尔金，Y. N.；索洛维耶娃，T. F.；奥沃多夫，Y. S. 《生物有机化学》1987年，13卷，1428 - 1429页]。 --> 2） - β - D - Qui3NAcyl - （1 --> 3） - α - L - Rhap - （1 --> 3） - α - D - FucpNAc - （1 --> 1. --> 4） - β - D - Qui3NAcyl - （1 --> 3） - α - L - 6dTalp4Ac - （1 --> 3） - α - D - FucpNAc - （1 --> 2.
