[重组海葵细胞毒素对大鼠血管平滑肌细胞增殖的影响]
[Effects of recombinant sea anemone cytotoxin on vascular smooth muscle cell proliferation in rats].
作者信息
Ouyang Ping, Jiang Xiao-Yu, Yang Wen-Li, Xu An-Long
机构信息
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou, 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2002 Nov;22(11):994-5.
OBJECTIVE
To observe the effects of recombined sea anemone cytotoxin (rSAC) on vascular smooth muscle cell proliferation in rats rSAC.
METHODS
Aortic smooth muscle cells (ASMCs) of rats were cultured in vitro and stimulated with rSAC of different concentrations, and were observed in comparison with the control group. The ratio of cell proliferation was determined with non-radioactive MTS/PES assay.
RESULTS
The ratio of cell proliferation rate was 0.802+/-0.055 in the control group, and was 0.115+/-0.014, 0.213+/-0.016, 0.335+/-0.097, 0.663+/-0.060, 0.678+/-0.129, and 0.738+/-0.072 in rSAC groups corresponding to rSAC concentrations of 100, 10, 1 mg/ml and 100, 10, 1 ng/ml respectively. It was shown that rSAC significantly inhibited the cell proliferation when the concentration used was above 100 ng/mg (P<0.05), in a dose-dependent manner as compared with the control group (P<0.05).
CONCLUSION
rSAC can significantly inhibit the vascular smooth muscle cell proliferation in rats.
目的
观察重组海葵细胞毒素（rSAC）对大鼠血管平滑肌细胞增殖的影响。
方法
体外培养大鼠主动脉平滑肌细胞（ASMCs），用不同浓度的rSAC刺激，并与对照组进行比较观察。采用非放射性MTS/PES法测定细胞增殖率。
结果
对照组细胞增殖率为0.802±0.055，rSAC浓度分别为100、10、1mg/ml和100、10、1ng/ml的rSAC组细胞增殖率分别为0.115±0.014、0.213±0.016、0.335±0.097、0.663±0.060、0.678±0.129和0.738±0.072。结果表明，当rSAC浓度高于100ng/mg时，可显著抑制细胞增殖（P<0.05），与对照组相比呈剂量依赖性（P<0.05）。
结论
rSAC可显著抑制大鼠血管平滑肌细胞增殖。
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