Reber Kristina M, Su Baogen Y, Clark K Reed, Pohlman Dana L, Miller Charles E, Nowicki Philip T
Department of Pediatrics, Ohio State University and the Children's Research Institute, Children's Hospital, Columbus, Ohio 43205, USA.
Am J Physiol Gastrointest Liver Physiol. 2002 Dec;283(6):G1328-35. doi: 10.1152/ajpgi.00067.2002.
Developmental changes in the expression of endothelial nitric oxide synthase (eNOS) within the mesenteric artery of swine were studied in fetal (110 days postconception/117 days total gestation) and on postnatal days 1, 3, 10, and 30. Subjects in the 1-day-old group were subdivided into fed and nonfed. Transcription of eNOS was determined by real-time PCR, protein expression was evaluated by Western blotting, and hemodynamic and oxygenation parameters were measured within in situ gut loops before and after the administration of N(G)-monomethyl-L-arginine (L-NMMA). The abundance of eNOS mRNA remained steady throughout all ages. In contrast, expression of eNOS protein was twofold greater in the 1-day-old fed subjects compared with fetal or 1-day-old nonfed subjects. eNOS protein expression remained elevated on day 3, increased on day 10, and then declined to a level similar to the day 1 nonfed group by postnatal day 30. Intestinal vascular resistance was 31% lower in the day 1 fed group when compared to the day 1 nonfed group; resistance continued to decline through day 10 but then significantly increased on day 30. We conclude that the expression of eNOS changes within the mesenteric artery during early postnatal development at a posttranscriptional level.
研究了猪肠系膜动脉中内皮型一氧化氮合酶（eNOS）表达的发育变化，研究对象为胎儿（受孕后110天/总妊娠期117天）以及出生后第1、3、10和30天的仔猪。将1日龄组的仔猪分为喂食组和未喂食组。通过实时PCR测定eNOS的转录，通过蛋白质印迹法评估蛋白质表达，并在给予N（G）-单甲基-L-精氨酸（L-NMMA）前后，在原位肠袢中测量血流动力学和氧合参数。eNOS mRNA的丰度在所有年龄段均保持稳定。相比之下，1日龄喂食仔猪的eNOS蛋白表达比胎儿或1日龄未喂食仔猪高出两倍。eNOS蛋白表达在第3天仍保持升高，在第10天增加，然后在出生后第30天降至与1日龄未喂食组相似的水平。与1日龄未喂食组相比，1日龄喂食组的肠道血管阻力降低了31%；阻力在第10天持续下降，但在第30天显著增加。我们得出结论，在出生后早期发育过程中，肠系膜动脉中eNOS的表达在转录后水平发生变化。
