Payne W A, Page R C, Ogilvie A L, Hall W B
J Periodontal Res. 1975 May;10(2):51-64. doi: 10.1111/j.1600-0765.1975.tb00008.x.
The pathologic alterations occurring in the gingival tissues of humans immediately following the beginning of plaque accumulation have not heen elucidated previously. Seven males, 22--31 years of age, free of clinical manifestations of dental and periodontal disease, and exhibiting a Plaque Index score of zero for the previous 28 days served as subjects. Plaque control measures were discontinued for 8 days and biopsies were taken from the buccal marginal gingiva of the first premolars on days 0, 2, 4, and 8. Paraffin- and Epon-embedded section, treated with a variety of histochemical stains were analyzed microscopically and cell counts were done on 1-micron Epon section. At 2 and 4 days following the beginning of plaque accumulation, the vessels subjacent to the juctional epithelium exhibited vasculitis and alterations in the perivascular collagen. There was a statistically significant increase in the number of neutrophils residing in the junctional epithelium. By the end of the 8-day period, the number of small mononuclear cells, mostly lymphocytes, in the connective tissues had increased by 3-fold and the area of collagen fiber alteration by 4-fold. In addition, the number of fibroblasts per unit area of connective tissue decreased significantly. Thus, within the period of 8 days following the beginning of plaque accumulation, an early lesion exhibiting many features characteristic of delayed hypersensitivity develops.
人类牙龈组织在菌斑开始积聚后立即发生的病理改变此前尚未阐明。7名年龄在22至31岁之间、无牙齿和牙周疾病临床表现且在过去28天菌斑指数评分为零的男性作为研究对象。菌斑控制措施中断8天，并在第0、2、4和8天从第一前磨牙的颊侧边缘龈取活检组织。对石蜡包埋和环氧树脂包埋的切片进行多种组织化学染色后进行显微镜分析，并在1微米厚的环氧树脂切片上进行细胞计数。在菌斑开始积聚后的第2天和第4天，结合上皮下方的血管出现血管炎和血管周围胶原改变。结合上皮内中性粒细胞数量有统计学意义的增加。到8天结束时，结缔组织中小单核细胞（主要是淋巴细胞）数量增加了3倍，胶原纤维改变面积增加了4倍。此外，结缔组织单位面积内的成纤维细胞数量显著减少。因此，在菌斑开始积聚后的8天内，会出现一种表现出许多迟发型超敏反应特征的早期病变。
