Biselli R, Ferlini C, Di Murro C, Paolantonio M, Fattorossi A
Laboratorio di Immunologia, Rep. Medicina, D.A.S.R.S., Aeroporto Pratica di Mare, Roma, Italy.
Inflammation. 1995 Aug;19(4):479-87. doi: 10.1007/BF01534581.
In gingival pockets of patients with periodontal disease, polymorphonuclear leukocytes (PMN) are in contact with a peculiar exudate, the gingival crevicular fluid (GCF). Because of the pivotal role played by PMN in periodontal disease, we evaluated the ability of GCF in modulating normal human PMN. GCF was obtained from two gingival sites with severe periodontitis (SP) and two gingival sites with only mild periodontitis (MP) in 12 patients. Purified PMN were exposed to GCF from SP and MP sites and, as a control, to sterile culture medium. GCF activity was evaluated by monitoring the modulation of membrane molecules relevant to cell function. Compared to control medium, GCF from SP and MP sites was able to induce an activation status in PMN evidenced by an increased CD11b (62 +/- 9% and 28 +/- 7%, respectively) and f-Met-Leu-Phe (56 +/- 5% and 31 +/- 7%, respectively) receptor expression, with a concomitant reduction of CD62L expression (56 +/- 8% and 23 +/- 7%, respectively). Thus, reflecting the clinical status, GCF from SP sites was significantly more efficient in affecting PMN than GCF from MP sites. Cell size modifications, evaluated as an additional indicator of PMN activation, were consistent with membrane molecule modulation. The difference in PMN-activating capacity between SP and MP was abrogated by the successful completion of an appropriate periodontal therapy that dramatically improved clinical status. This is the first direct demonstration that GCF from periodontitis has the capacity to activate normal resting PMN and that this capacity reflects the magnitude of the inflammatory process that takes place in the gingiva.
在牙周病患者的牙龈袋中，多形核白细胞（PMN）与一种特殊的渗出物——龈沟液（GCF）接触。由于PMN在牙周病中发挥着关键作用，我们评估了GCF调节正常人PMN的能力。从12例患者的两个重度牙周炎（SP）牙龈部位和两个仅轻度牙周炎（MP）牙龈部位获取GCF。将纯化的PMN暴露于来自SP和MP部位的GCF，并作为对照暴露于无菌培养基。通过监测与细胞功能相关的膜分子的调节来评估GCF活性。与对照培养基相比，来自SP和MP部位的GCF能够诱导PMN的激活状态，表现为CD11b受体表达增加（分别为62±9%和28±7%）以及f-Met-Leu-Phe受体表达增加（分别为56±5%和31±7%），同时CD62L表达降低（分别为56±8%和23±7%）。因此，反映临床状况的是，来自SP部位的GCF比来自MP部位的GCF在影响PMN方面效率显著更高。作为PMN激活的另一个指标评估的细胞大小变化与膜分子调节一致。通过成功完成适当的牙周治疗显著改善临床状况后，SP和MP之间PMN激活能力的差异消除。这是首次直接证明来自牙周炎的GCF有能力激活正常静息的PMN，并且这种能力反映了牙龈中发生的炎症过程的程度。
