Hayes Richard B, Smith Craig O, Huang Wen-Yi, Read Yvonne, Kopp William C
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland 20892, USA.
Cancer Epidemiol Biomarkers Prev. 2002 Nov;11(11):1496-8.
Standardized and cost-effective biological sample collection, processing, and storage procedures are needed in large-scale epidemiological studies to provide material for testing a broad range of etiological hypotheses. One component of sample collection in the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial involves shipment of blood in acid-citrate-dextrose anticoagulant to a central processing laboratory, where 10% DMSO is added, and whole blood aliquots are cryopreserved. A single technician is able to routinely process 50-60 samples/day. Tests conducted to evaluate potential uses of cryopreserved whole blood showed successful EBV transformation (>90%, up to 20 months of storage). In addition, lymphocytes maintained good viability and stable T-cell:B-cell ratios, and T cells maintained the capacity to proliferate in response to solid phase anti-CD3/CD28 plus interleukin 2. Whole blood cryopreservation is a cost-effective approach to large-scale storage of viable cells in epidemiological studies.
在大规模流行病学研究中，需要标准化且具有成本效益的生物样本采集、处理和储存程序，以便为检验广泛的病因假设提供材料。前列腺、肺癌、结直肠癌和卵巢癌筛查试验中的样本采集的一个环节是，将用柠檬酸葡萄糖抗凝的血液运送到中央处理实验室，在那里添加10%的二甲基亚砜，并将全血 aliquots 冷冻保存。一名技术人员每天能够常规处理50 - 60个样本。为评估冷冻保存全血的潜在用途而进行的测试表明，EBV 转化成功（>90%，储存长达20个月）。此外，淋巴细胞保持了良好的活力和稳定的 T 细胞与 B 细胞比例，并且 T 细胞保持了对固相抗 CD3/CD28 加白细胞介素 2 作出增殖反应的能力。全血冷冻保存是在流行病学研究中大规模储存活细胞的一种具有成本效益的方法。
