Kristal Alan R, King Irena B, Albanes Demetrius, Pollak Michael N, Stanzyk Frank Z, Santella Regina M, Hoque Ashraful
Cancer Prevention Program, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
Cancer Epidemiol Biomarkers Prev. 2005 Mar;14(3):727-30. doi: 10.1158/1055-9965.EPI-04-0596.
This experiment examined the effects of delays in separation and freezing of whole blood components on analytes of interest in studies of prostate cancer prevention, in order to evaluate the feasibility of centralized processing of blood for the multisite Selenium and Vitamin E Cancer Prevention Trial. Blood from 40 healthy men was subjected to four treatment protocols, allowing the contrast of immediate processing to delays of 32, 72, and 144 hours. At 32 hours, simulating refrigerated storage and overnight shipping, there was a 2.9% decrease (95% confidence interval, 0.7-5.1) in insulin-like growth factor-I (IGF-I) but no significant change in carotenoids, tocopherols, testosterone, 3alpha-androstanediol glucuronide (AAG), sex hormone-binding globulin (SHBG) or insulin-like growth factor binding protein 3 (IGFBP3). A 144-hour processing delay, simulating weekend blood collection or shipping delay, resulted in significant changes in gamma-tocopherol (-1.5%), IGF-I (-5.7%), IGFBP3 (-2.9%), SHBG (-4.0%), testosterone (+4.7%), and AAG (+5.5%). The rank-order and intraclass correlations between analytes from blood processed immediately and those subjected to delayed processing were 0.96 or higher for carotenoids, tocopherols, AAG, and SHBG, and between 0.87 and 0.95 for IGF-I, IGFBP3, and testosterone. A 32-hour delay decreased lymphocyte viability from 82.5% to 75.0% (P = 0.45), but a 72-hour delay decreased viability to 36.8% (P < 0.001). Overnight shipping and centralized processing is an acceptable approach to blood collection in large multisite trials examining the cancer-related measures proposed in the Selenium and Vitamin E Cancer Prevention Trial. Longer processing delays, however, have small but statistically significant effects on many analytes and substantially decrease lymphocyte viability.
本实验研究了全血成分分离和冷冻延迟对前列腺癌预防研究中相关分析物的影响，以评估在多中心硒与维生素E癌症预防试验中进行血液集中处理的可行性。40名健康男性的血液接受了四种处理方案，以便对比即时处理与延迟32、72和144小时后的情况。在32小时时，模拟冷藏储存和隔夜运输，胰岛素样生长因子-I（IGF-I）下降了2.9%（95%置信区间，0.7 - 5.1），但类胡萝卜素、生育酚、睾酮、3α - 雄烷二醇葡萄糖醛酸苷（AAG）、性激素结合球蛋白（SHBG）或胰岛素样生长因子结合蛋白3（IGFBP3）无显著变化。144小时的处理延迟，模拟周末采血或运输延迟，导致γ - 生育酚（-1.5%）、IGF-I（-5.7%）、IGFBP3（-2.9%）、SHBG（-4.0%）、睾酮（+4.7%）和AAG（+5.5%）出现显著变化。即时处理的血液与延迟处理的血液中分析物之间的秩次顺序和组内相关性，类胡萝卜素、生育酚、AAG和SHBG为0.96或更高，IGF-I、IGFBP3和睾酮为0.87至0.95。32小时的延迟使淋巴细胞活力从82.5%降至75.0%（P = 0.45），但72小时的延迟使活力降至36.8%（P < 0.001）。在大型多中心试验中，隔夜运输和集中处理是采集血液的一种可接受方法，此类试验旨在研究硒与维生素E癌症预防试验中提出的癌症相关指标。然而，较长的处理延迟对许多分析物有虽小但具有统计学意义的影响，并显著降低淋巴细胞活力。
