布卢姆综合征解旋酶可刺激人类拓扑异构酶IIIα的活性。
The Bloom's syndrome helicase stimulates the activity of human topoisomerase IIIalpha.
作者信息
Wu Leonard, Hickson Ian D
机构信息
Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK.
出版信息
Nucleic Acids Res. 2002 Nov 15;30(22):4823-9. doi: 10.1093/nar/gkf611.
Abstract
Bloom's syndrome (BS) is a disorder associated with chromosomal instability and a predisposition to the development of cancer. The BS gene product, BLM, is a DNA helicase of the RecQ family that forms a complex in vitro and in vivo with topoisomerase IIIalpha. Here, we show that BLM stimulates the ability of topoisomerase IIIalpha to relax negatively supercoiled DNA. Moreover, DNA binding analyses indicate that BLM recruits topoisomerase IIIalpha to its DNA substrate. Consistent with this, a mutant form of BLM that retains helicase activity, but is unable to bind topoisomerase IIIalpha, fails to stimulate topoisomerase activity. These results indicate that a physical association between BLM and topoisomerase IIIalpha is a prerequisite for their functional biochemical interaction.
摘要
布卢姆综合征（BS）是一种与染色体不稳定以及易患癌症相关的疾病。BS基因产物BLM是RecQ家族的一种DNA解旋酶，它在体外和体内都能与拓扑异构酶IIIα形成复合物。在此，我们表明BLM能刺激拓扑异构酶IIIα使负超螺旋DNA松弛的能力。此外，DNA结合分析表明BLM将拓扑异构酶IIIα招募到其DNA底物上。与此一致的是，一种保留解旋酶活性但无法结合拓扑异构酶IIIα的BLM突变形式不能刺激拓扑异构酶活性。这些结果表明BLM与拓扑异构酶IIIα之间的物理关联是它们功能性生化相互作用的先决条件。
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1
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Cold Spring Harb Symp Quant Biol. 2000;65:573-81. doi: 10.1101/sqb.2000.65.573.
2
Generation of double-stranded breaks in hypernegatively supercoiled DNA by Drosophila topoisomerase IIIbeta, a type IA enzyme.果蝇拓扑异构酶IIIβ（一种IA型酶）在超负超螺旋DNA中产生双链断裂。
J Biol Chem. 2002 Jul 26;277(30):26865-71. doi: 10.1074/jbc.M204641200. Epub 2002 May 22.
3
Topoisomerase III acts upstream of Rad53p in the S-phase DNA damage checkpoint.拓扑异构酶III在S期DNA损伤检查点中作用于Rad53p的上游。
Mol Cell Biol. 2001 Nov;21(21):7150-62. doi: 10.1128/MCB.21.21.7150-7162.2001.
4
Association of yeast DNA topoisomerase III and Sgs1 DNA helicase: studies of fusion proteins.酵母DNA拓扑异构酶III与Sgs1解旋酶的关联：融合蛋白研究
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11108-13. doi: 10.1073/pnas.201387098. Epub 2001 Sep 11.
5
RecQ helicases and topoisomerases: components of a conserved complex for the regulation of genetic recombination.RecQ解旋酶与拓扑异构酶：调控基因重组的保守复合物的组成成分
Cell Mol Life Sci. 2001 Jun;58(7):894-901. doi: 10.1007/PL00000909.
6
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7
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8
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9
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10
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