Bizard Anna H, Hickson Ian D
Nordea Center for Healthy Aging, Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, 2200 Copenhagen N, Denmark.
Cold Spring Harb Perspect Biol. 2014 Jul 1;6(7):a016477. doi: 10.1101/cshperspect.a016477.
Double Holliday junctions (dHJS) are important intermediates of homologous recombination. The separate junctions can each be cleaved by DNA structure-selective endonucleases known as Holliday junction resolvases. Alternatively, double Holliday junctions can be processed by a reaction known as "double Holliday junction dissolution." This reaction requires the cooperative action of a so-called "dissolvasome" comprising a Holliday junction branch migration enzyme (Sgs1/BLM RecQ helicase) and a type IA topoisomerase (Top3/TopoIIIα) in complex with its OB (oligonucleotide/oligosaccharide binding) fold containing accessory factor (Rmi1). This review details our current knowledge of the dissolution process and the players involved in catalyzing this mechanistically complex means of completing homologous recombination reactions.
双Holliday连接体（dHJS）是同源重组的重要中间体。单个连接体均可被称为Holliday连接体解离酶的DNA结构选择性核酸内切酶切割。另外，双Holliday连接体可通过一种称为“双Holliday连接体溶解”的反应进行处理。该反应需要一种所谓“溶解体”的协同作用，该溶解体由一个Holliday连接体分支迁移酶（Sgs1/BLM RecQ解旋酶）和一个IA型拓扑异构酶（Top3/TopoIIIα）与其包含辅助因子（Rmi1）的OB（寡核苷酸/寡糖结合）折叠结构形成复合物组成。本综述详细阐述了我们目前对溶解过程以及参与催化这种机制复杂的完成同源重组反应方式的相关因子的了解。
