Barrett Anne, Magee John G, Freeman Roger
North of England Regional Centre for Mycobacteriology, Public Health Laboratory, General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE.
J Med Microbiol. 2002 Oct;51(10):895-898. doi: 10.1099/0022-1317-51-10-895.
In controlling the spread of tuberculosis, early detection of disease caused by organisms of the Mycobacterium tuberculosis complex (MTBC) is vital. The BD ProbeTec ET system provides a method for the direct detection of MTBC by strand displacement amplification. Two hundred and five respiratory samples from patients with a high probability of tuberculosis were assessed by ProbeTec and by microscopy and culture for mycobacteria. ProbeTec positive results were obtained with 101 of 109 samples from which MTBC organisms were isolated. ProbeTec correctly signalled 78 of 81 samples that gave growths of mycobacteria other than tubercle bacilli (MOTT) as negative. Three samples gave false-positive results, corrected on repeat testing. Positive and negative predictive values (PPV, NPV) were 0.97 and 0.90 and the system showed a sensitivity and specificity of 92.7% and 96.0%, respectively. These values rose to PPV 0.97, NPV 0.96, sensitivity 97.1% and specificity 96.0% when data from the small number of gastric lavage samples tested were removed from the analysis. The BD ProbeTec ET system offers a robust and reliable molecular biological approach to the detection of MTBC organisms in respiratory samples in a semi-automated format.
在控制结核病传播方面，早期检测由结核分枝杆菌复合群（MTBC）病原体引起的疾病至关重要。BD ProbeTec ET系统提供了一种通过链置换扩增直接检测MTBC的方法。对205份极有可能患有结核病的患者的呼吸道样本进行了ProbeTec检测，并通过显微镜检查和分枝杆菌培养进行评估。在从109份样本中分离出MTBC病原体的样本中，有101份获得了ProbeTec阳性结果。ProbeTec正确地将81份培养出非结核杆菌（MOTT）的分枝杆菌的样本中的78份判定为阴性。有3份样本出现假阳性结果，重复检测后得到纠正。阳性和阴性预测值（PPV、NPV）分别为0.97和0.90，该系统的灵敏度和特异度分别为92.7%和96.0%。当从分析中去除少量检测的洗胃样本的数据后，这些值上升到PPV 0.97、NPV 0.96、灵敏度97.1%和特异度96.0%。BD ProbeTec ET系统以半自动形式为呼吸道样本中MTBC病原体的检测提供了一种强大且可靠的分子生物学方法。
