Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Sensors (Basel). 2012;12(6):8319-37. doi: 10.3390/s120608319. Epub 2012 Jun 14.
Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.
自从聚合酶链反应 (PCR) 技术发展以来，与以前相比，可以从更少的 DNA 中获取基因组信息。基于 PCR 的扩增需要高精度的仪器来进行温度循环反应; 此外，它们对于常规临床使用来说很繁琐。然而，使用等温方法可以消除与热循环相关的许多并发症。最近，人们广泛研究了用于等温 DNA 扩增的诊断设备的应用。在本文中，我们描述了几种等温扩增方法的基本概念，并回顾了诊断设备开发的最新进展。
