Chen M, Aosai F, Norose K, Mun H S, Takeuchi O, Akira S, Yano A
Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Japan.
J Parasitol. 2002 Oct;88(5):1017-9. doi: 10.1645/0022-3395(2002)088[1017:IOMIHD]2.0.CO;2.
This study investigated the influence of TLR (toll-like receptor)4, TLR2, and MyD88 in Toxoplasma gondii-infected wild-type (WT) mice and TLR4-, TLR2-, and MyD88-deficient mice. Ninety-five percent of MyD88-deficient mice died 10-16 days after intraperitoneal infection with 100 cysts of T. gondii Fukaya strain, whereas 95-100% of TLR4- and TLR2-deficient mice and WT C57BL/6 (B6) mice survived for more than 7 wk after T. gondii infection. The distribution of T. gondii in various organs of TLR4-, TLR2-, and MyD88-deficient mice and WT B6 mice was assessed 2 wk after T. gondii intraperitoneal infection using quantitative competitive polymerase chain reaction. In MyD88-deficient mice, high levels of T. gondii load were observed in the brain, tongue, heart, lungs, spleen, liver, mesenteric lymph node, and kidneys after infection. The T. gondii load was significantly increased in the lungs in both TLR4- and TLR2-deficient mice compared with WT B6 mice. High levels of anti-mouse heat shock protein (mHSP)70 autoantibody and anti-T. gondii HSP70 antibody production were detected in the sera from MyD88-deficient mice.
本研究调查了Toll样受体（TLR）4、TLR2和髓样分化因子88（MyD88）在刚地弓形虫感染的野生型（WT）小鼠以及TLR4、TLR2和MyD88基因缺陷小鼠中的作用。95%的MyD88基因缺陷小鼠在腹腔感染100个刚地弓形虫深谷株包囊后10 - 16天死亡，而95 - 100%的TLR4和TLR2基因缺陷小鼠以及野生型C57BL/6（B6）小鼠在刚地弓形虫感染后存活超过7周。在刚地弓形虫腹腔感染2周后，采用定量竞争聚合酶链反应评估TLR4、TLR2和MyD88基因缺陷小鼠以及野生型B6小鼠各器官中刚地弓形虫的分布。在MyD88基因缺陷小鼠中，感染后在脑、舌、心脏、肺、脾脏、肝脏、肠系膜淋巴结和肾脏中观察到高水平的刚地弓形虫载量。与野生型B6小鼠相比，TLR4和TLR2基因缺陷小鼠肺中的刚地弓形虫载量均显著增加。在MyD88基因缺陷小鼠的血清中检测到高水平的抗小鼠热休克蛋白（mHSP）70自身抗体和抗刚地弓形虫HSP70抗体。
