Peng Jialing, Lin Xiancan, Lin Hongchun, Chen Shengjie, Liu Jinfeng, Guo Zexin, Liang Yuqing, Huang Shiguang, Lu Fangli
Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Parasitol Res. 2016 Dec;115(12):4681-4686. doi: 10.1007/s00436-016-5226-8. Epub 2016 Aug 17.
Toll-like receptors (TLRs) play a central role in the pathogen clearance and pathological processes. The liver is an important innate immune organ, in which Kupffer cells and hepatocytes are important innate immune cells. However, the role of TLR2 and TLR4 in the liver caused by Toxoplasma gondii infection remains less clear. In this study, mice were infected with T. gondii RH strain and the grades of liver and spleen injuries were histopathologically evaluated. TLR2 and TLR4 cells in the livers and spleens were detected by immunohistochemistry, and their messenger RNA (mRNA) expressions were detected using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). The pathological severities in the livers and spleens were increased with time in T. gondii-infected mice. Compared with uninfected controls, obvious TLR2 and TLR4 cells were observed in the livers and spleens infected with T. gondii at 8 days post-infection, accompanied with significantly over-expressed mRNA levels of TLR2 and TLR4 in the livers and spleens after infection. Our data indicated that increased levels of TLR2 and TLR4 in the liver and spleen may play an important role during acute T. gondii infection.
Toll样受体（TLRs）在病原体清除和病理过程中发挥着核心作用。肝脏是重要的天然免疫器官，其中库普弗细胞和肝细胞是重要的天然免疫细胞。然而，弓形虫感染所致肝脏中TLR2和TLR4的作用仍不太清楚。在本研究中，用弓形虫RH株感染小鼠，并对肝脏和脾脏损伤程度进行组织病理学评估。通过免疫组织化学检测肝脏和脾脏中的TLR2和TLR4细胞，并用定量实时逆转录-聚合酶链反应（qRT-PCR）检测其信使核糖核酸（mRNA）表达。弓形虫感染小鼠的肝脏和脾脏病理严重程度随时间增加。与未感染对照组相比，感染后8天在感染弓形虫的肝脏和脾脏中观察到明显的TLR2和TLR4细胞，且感染后肝脏和脾脏中TLR2和TLR4的mRNA水平显著过度表达。我们的数据表明，肝脏和脾脏中TLR2和TLR4水平升高可能在急性弓形虫感染过程中起重要作用。
