PP01宿主范围突变体的Gp38中的氨基酸改变及其感染大肠杆菌O157:H7的ompC缺失突变体的证据。
Amino acid alterations in Gp38 of host range mutants of PP01 and evidence for their infection of an ompC null mutant of Escherichia coli O157:H7.
作者信息
Morita Masatomo, Fischer Curt R, Mizoguchi Katsunori, Yoichi Masatoshi, Oda Masahito, Tanji Yasunori, Unno Hajime
机构信息
Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
出版信息
FEMS Microbiol Lett. 2002 Nov 5;216(2):243-8. doi: 10.1111/j.1574-6968.2002.tb11442.x.
The previously isolated T-even type coliphage PP01, specifically infective to Escherichia coli O157:H7, uses the outer membrane protein OmpC as a receptor. The characterization of a spontaneous PP01-resistant strain indicated that it had lost ompC due to the deletion of a 14-kbp region upstream of and partially including ompC. Two host range mutants, able to infect an ompC null mutant, were isolated. Sequencing of gene 38, which codes for the receptor recognition protein Gp38, indicated three mutations in one mutant and two in the other. Both mutant proteins had a Gly208Arg, a Gly161Arg or Gly101His replacement, respectively, and the one mutant phage in addition a Trp189Arg replacement. These alterations suggest that the host range was mediated by a more positively charged Gp38.
先前分离出的对大肠杆菌O157:H7具有特异性感染性的T偶数型大肠杆菌噬菌体PP01，将外膜蛋白OmpC用作受体。一株自发的PP01抗性菌株的特征表明，由于ompC上游14kbp区域的缺失（部分包括ompC），它失去了ompC。分离出了两个能够感染ompC缺失突变体的宿主范围突变体。编码受体识别蛋白Gp38的基因38测序表明，一个突变体中有三个突变，另一个中有两个突变。两种突变蛋白分别有Gly208Arg、Gly161Arg或Gly101His替代，并且其中一个突变噬菌体还有Trp189Arg替代。这些改变表明宿主范围是由带更多正电荷的Gp38介导的。
Zotero 插件