[蓝噬菌体LPP - 3的DNA物理图谱]
[Physical mapping of DNA of cyanophage LPP-3].
作者信息
Syrchin S A, Mendzhul M I
机构信息
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
出版信息
Mikrobiol Z. 2002 Jul-Aug;64(4):24-30.
Restrictases fit for the purposes of physical mapping of cyanophage LPP-3 DNA have been selected as a result of the restriction analysis. The use of the methods of mutual hydrolysis, restriction of the fragment isolated from gel and terminal labeling allowed formation a physical map of LPP-3 cyanophage DNA with the complete scheme of allocation of 14 sites for 8 restrictases: Alw44I, Bsp1191, BsuRI, Eco147I, EheI, NcoI, Kpn2I and PvuI as well as the position of certain sites for restrictases HindIII, KpnI and Sau3A.
通过限制性分析，已筛选出适用于蓝噬菌体LPP - 3 DNA物理图谱构建的限制性内切酶。相互水解法、从凝胶中分离片段的限制性内切酶消化以及末端标记法的应用，使得构建出了LPP - 3蓝噬菌体DNA的物理图谱，该图谱完整呈现了8种限制性内切酶（Alw44I、Bsp1191、BsuRI、Eco147I、EheI、NcoI、Kpn2I和PvuI）的14个酶切位点的分布情况，以及限制性内切酶HindIII、KpnI和Sau3A某些酶切位点的位置。
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