Kul'ba A M, Gorelyshev A S, Fomichev Iu K
Mol Biol (Mosk). 1986 Jan-Feb;20(1):181-4.
The DNA of temperate phage SM P. aeruginosa has one PvuII site, two BamHI sites, three HindIII sites and five EcoRI sites. Using these restrictases the physical map of the phage genome has been constructed. The DNA of phage SM has in their structure cohesive ends similar to cos-sites of phage lambda DNA. EcoRI-fragments with cohesive ends have molecular masses 2.9 and 4.9 MDa.
温和噬菌体铜绿假单胞菌SM的DNA有一个PvuII位点、两个BamHI位点、三个HindIII位点和五个EcoRI位点。利用这些限制性内切酶构建了噬菌体基因组的物理图谱。噬菌体SM的DNA在其结构上具有与噬菌体λDNA的粘性末端类似的粘性末端。具有粘性末端的EcoRI片段的分子量分别为2.9和4.9兆道尔顿。
