Zaĭchenko O M, Mendzhul M I, Lysenko T H, Andriienko O V, Shaïns'ka O O, Busakhina I V
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mikrobiol Z. 2002 Jul-Aug;64(4):31-9.
Screening of cyanobacteria cultures for their sensitivity to the action of complex preparations of toxins from 18 strains of Stachybotrys chartarum has been carried out with the purpose to search for new test-organisms to create a reliable system of biotesting of stachibotryotoxines. In contrast to other cultures of unicell (Anacystis nidulans) and filamentous (Anabaena sp., Anabaena variabilis, Nostoc linckia, Plectonema boryanum) cyanobacteria used in the experiment, only one culture of unicell cyanobacterium Synechococcus cedrorum proved to be highly sensitive to the action of preparations of the whole group of studied mycotoxins. Their activity with respect to this culture varied within wide limits (lysis zone diameter is 11-30 mm) and for the most of strains it coincided with or exceeded this index determined with the use of other biotests. Owing to the use of Synechococcus cedrorum as test culture the authors have proved for the first time the toxicity of three strains of Stachybotrys chartarum (K15822, 14722, 14186). Basing on the data obtained the authors have determined the culture of the unicell cyanobacterium, according to the sign of high sensitivity to all the studied stachibotryotoxin preparations of solid and liquid nutrient media, as a new test-organism for detection of this group of toxins and it may be recommended for practical use. Optimal conditions for growing test-culture and biotesting of toxins have been defined.
