Nummi N, Niku-Paavola M L
Ann Nutr Aliment. 1977;31(4-6):761-70.
To attempt to clarify the mechanism of the action of mycotoxins under physiological conditions, the effect of simulated gastrointestinal conditions on the toxicity of Stachybotrys alternans was studied. Components, extracted into a simulated gastrointestinal system and into organic solvents were compared, regarding toxicity and chromatographic properties, with particular reference to a chromatographically characterized Stachybotrys-toxin found by us. It was established that, in addition to those toxic components which were soluble in organic solvents, some toxic, highly water soluble substances were extracted from the fungal culture by the gastrointestinal system. The total toxicity extracted by organic solvents was about fifteen times as much as the toxicity extracted by the simulated system, as measured by cell toxicity tests. All of the toxicity can be transferred from the fungus culture to the aqueous simulated system. This means that the simulated system altered the nature of the toxic components extractible with organic solvents.
为了试图阐明霉菌毒素在生理条件下的作用机制，研究了模拟胃肠道条件对链格孢菌毒性的影响。将提取到模拟胃肠道系统和有机溶剂中的成分，就毒性和色谱性质进行比较，特别参照我们发现的一种经色谱表征的链格孢毒素。结果表明，除了那些可溶于有机溶剂的有毒成分外，胃肠道系统还从真菌培养物中提取了一些有毒的、高度水溶性的物质。通过细胞毒性试验测定，有机溶剂提取的总毒性约为模拟系统提取毒性的15倍。所有的毒性都可以从真菌培养物转移到水相模拟系统中。这意味着模拟系统改变了可用有机溶剂提取的有毒成分的性质。
