Schmid Heide, Sauerbrei Ramona, Schwarz Gerold, Weber Ekkehard, Kalbacher Hubert, Driessen Christoph
Institut für Pathologie, Universitätsklinikum Tübingen, Germany.
Biol Chem. 2002 Jul-Aug;383(7-8):1277-83. doi: 10.1515/BC.2002.143.
Endosomal and lysosomal fractions of human monocytes/macrophages prepared from buffy coats were analyzed for activities of cathepsins B, L and S, and expression of cathepsin proteins along with major histocompatibility complex class I and class II molecules under control and immunomodulatory conditions. While the total activity of cathepsins B, L, and S together remained unchanged in lysates of control cells during culture for 72 h, the subcellular distribution of cathepsin activities underwent a shift from a predominantly endosomal localization in freshly isolated cells to a lysosomal pattern after 72 h of culture. Interferon-gamma treatment for 72 h resulted in an upregulation of both major histocompatibility complex proteins and cathepsins with differential changes in cathepsin B, L and S activities in endosomes versus lysosomes. These changes suggest a remodeling of the endocytic machinery and imply different functions of cathepsins B, L and S during monocyte differentiation.
对从血沉棕黄层制备的人单核细胞/巨噬细胞的内体和溶酶体部分进行分析，检测组织蛋白酶B、L和S的活性，以及在对照和免疫调节条件下组织蛋白酶蛋白与主要组织相容性复合体I类和II类分子的表达。在对照细胞裂解物培养72小时期间，组织蛋白酶B、L和S的总活性保持不变，但组织蛋白酶活性的亚细胞分布发生了变化，从新鲜分离细胞中主要定位于内体转变为培养72小时后的溶酶体模式。用干扰素-γ处理72小时导致主要组织相容性复合体蛋白和组织蛋白酶均上调，内体与溶酶体中的组织蛋白酶B、L和S活性发生不同变化。这些变化表明内吞机制发生了重塑，意味着组织蛋白酶B、L和S在单核细胞分化过程中具有不同功能。
