A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-beta-lactam.

作者信息

Ostler Elizabeth L, Dean Christopher J, Barber Nicola, Valeri Maurizio, James Stuart, Resmini Marina, Boucher Guillaume, Romanov Nickolas, Brocklehurst Keith, Gallacher Gerard

机构信息

School of Pharmacy and Biomolecular Sciences, University of Brighton, Cockcroft Building, Moulsecoomb, BN2 4GJ, Brighton, UK.

出版信息

Biochem Biophys Res Commun. 2002 Nov 29;299(2):273-6. doi: 10.1016/s0006-291x(02)02604-9.

DOI:10.1016/s0006-291x(02)02604-9

PMID:12437982

Abstract

We report the first example of a monoclonal antibody-catalysed hydrolysis of a beta-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with k(cat)=8.7 x 10(-6)s(-1) and K(M)=35 microM. This is the first example of a rat monoclonal catalytic antibody.

摘要