Goertz David E, Yu Joanne L, Kerbel Robert S, Burns Peter N, Foster F Stuart
Sunnybrook and Women's College Health Sciences Centre, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, M4N 3M5 Canada.
Cancer Res. 2002 Nov 15;62(22):6371-5.
The effect of antivascular therapy on blood flow in superficial tumors was monitored using novel high frequency Doppler (HFD) ultrasound techniques. Human melanoma cells (MeWo) were injected orthotopically into the skin of athymic nude mice. Volumetric HFD imaging of established melanomas detected a significant reduction in blood flow 4 h after injection of the tumor vascular targeting agent ZD6126 followed by a recovery of flow by 24 h after injection. Measurements of tumor perfusion in situ by Hoechst 33342 staining correlated with the ultrasound results. This study demonstrates the feasibility of HFD as a noninvasive, quantitative tool for following longitudinally the effects of antivascular therapy on blood flow in superficial tumors.
使用新型高频多普勒（HFD）超声技术监测抗血管治疗对浅表肿瘤血流的影响。将人黑色素瘤细胞（MeWo）原位注射到无胸腺裸鼠的皮肤中。对已形成的黑色素瘤进行容积HFD成像检测到，注射肿瘤血管靶向剂ZD6126后4小时血流显著减少，随后在注射后24小时血流恢复。通过Hoechst 33342染色对肿瘤原位灌注进行的测量与超声结果相关。本研究证明了HFD作为一种无创、定量工具用于纵向跟踪抗血管治疗对浅表肿瘤血流影响的可行性。
