Sytnyk Vladimir, Leshchyns'ka Iryna, Delling Markus, Dityateva Galina, Dityatev Alexander, Schachner Melitta
Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany.
J Cell Biol. 2002 Nov 25;159(4):649-61. doi: 10.1083/jcb.200205098. Epub 2002 Nov 18.
Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin to clusters of the neural cell adhesion molecule (NCAM) in the plasma membrane. These complexes are translocated along neurites and trapped at sites of initial neurite-to-neurite contacts within several minutes after initial contact formation. The accumulation of TGN organelles at contacts with NCAM-deficient neurons is reduced when compared with wild-type cells, suggesting that NCAM mediates the anchoring of intracellular organelles in nascent synapses.
轴突与树突之间的接触转变为突触的过程伴随着突触机制在该部位的积累,这些机制主要由源自反式高尔基体网络(TGN)的细胞内细胞器传递。在此,我们报告在培养的海马神经元中,TGN细胞器通过血影蛋白与质膜中的神经细胞黏附分子(NCAM)簇相连。这些复合物沿神经突转运,并在最初接触形成后的几分钟内被困在神经突与神经突最初接触的部位。与野生型细胞相比,与缺乏NCAM的神经元接触时TGN细胞器的积累减少,这表明NCAM介导细胞内细胞器在新生突触中的锚定。